Bernutz C, Horn K, König A, Pickardt C R
J Clin Chem Clin Biochem. 1985 Dec;23(12):851-6. doi: 10.1515/cclm.1985.23.12.851.
Using two new immunoradiometric assays for thyrotropin, we measured thyrotropin levels in serum of patients suffering from various clinically and biochemically diagnosed thyroid disorders, and in healthy controls. A thyroliberin stimulation test was performed in all patients and controls. Thyrotropin levels were measured using a solid phase IRMA in 339 patients and a coated tube sandwich assay in 152 patients. The lower limits of detection of 0.1 mU/1 (solid phase IRMA) and 0.02 mU/1 (coated tube sandwich assay) as well as the specificity of these two assays were superior to those of conventional thyrotropin assays. Basal thyrotropin values were clearly different between euthyroid controls and patients non responding to thyroliberin stimulation. However, the values for these two groups overlapped those for patients showing a subnormal increase upon stimulation with thyroliberin. The thyroliberin stimulation test in patients with autonomous adenomas, together with the measurement of thyrotropin using these sensitive assays, provides additional information in the low range below 1.0 mU/1,i.e. below the lower limit of detection of conventional double antibody radioimmunoassays.
我们使用两种新的促甲状腺激素免疫放射分析方法，对患有各种临床和生化诊断甲状腺疾病的患者血清以及健康对照者血清中的促甲状腺激素水平进行了测量。对所有患者和对照者均进行了促甲状腺素释放激素刺激试验。在339例患者中使用固相免疫放射分析测量促甲状腺激素水平，在152例患者中使用包被管夹心分析。这两种分析方法的检测下限分别为0.1 mU/1（固相免疫放射分析）和0.02 mU/1（包被管夹心分析），且其特异性均优于传统促甲状腺激素分析方法。甲状腺功能正常的对照者与对促甲状腺素释放激素刺激无反应的患者之间，基础促甲状腺激素值明显不同。然而，这两组的值与促甲状腺素释放激素刺激后反应低于正常的患者的值有重叠。对于自主性腺瘤患者，促甲状腺素释放激素刺激试验以及使用这些敏感分析方法测量促甲状腺激素，在低于1.0 mU/1的低范围内，即低于传统双抗体放射免疫分析检测下限的范围内，可提供更多信息。
