Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil.
Institute of Biological and Health Sciences, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil.
Protein Expr Purif. 2025 Jan;225:106584. doi: 10.1016/j.pep.2024.106584. Epub 2024 Aug 22.
Lipases comprise the third most commercialized group of enzymes worldwide and those of microbial origin are sought for their multiple advantages. Agro-industrial waste can be an alternative culture medium for producing lipases, reducing production costs and the improper disposal of waste frying oil (WFO). This study aimed to produce yeast lipases through submerged fermentation (SF) using domestic edible oil waste as inducer and alternative culture medium. The optimal culture conditions, most effective inducer, and purification method for a new lipase from Moesziomyces aphidis BRT57 were identified. Yeast was cultured in medium containing green coconut pulp and WFO waste for 72 h. The maximum production of lipases in SF occurred in a culture medium containing WFO and yeast extract at 48 and 72 h of incubation, with enzyme activities of 8.88 and 11.39 U mL, respectively. The lipase was isolated through ultrafiltration followed by size exclusion chromatography, achieving a 50.46 % recovery rate. To the best of our knowledge, this is the first study to report the production and purification of lipases from M. aphidis, demonstrating the value of frying oil as inducer and alternative medium for SF, contributing to the production of fatty acids for biodiesel from food waste.
脂肪酶是全球商业化程度第三高的酶类，而微生物来源的脂肪酶因其多种优势而备受青睐。农业工业废物可以作为生产脂肪酶的替代培养基，降低生产成本，避免废食用油（WFO）的不当处理。本研究旨在通过深层发酵（SF）利用国产食用油废物作为诱导物和替代培养基来生产酵母脂肪酶。对从 Moesziomyces aphidis BRT57 中提取的新型脂肪酶的最佳培养条件、最有效诱导物和纯化方法进行了鉴定。酵母在含有绿椰果肉和 WFO 废物的培养基中培养 72 小时。在发酵 48 和 72 小时时，WFO 和酵母提取物含量分别为 48 和 72 小时的 SF 培养基中脂肪酶的最大产量分别为 8.88 和 11.39 U/mL。脂肪酶通过超滤和分子筛层析进行分离，回收率达到 50.46%。据我们所知，这是首次报道从 M. aphidis 中生产和纯化脂肪酶的研究，证明了煎炸油作为诱导物和 SF 替代培养基的价值，为从食物废物中生产生物柴油的脂肪酸做出了贡献。
