Silveira Erick A, Tardioli Paulo W, Farinas Cristiane S
Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970, São Carlos, SP, Brazil.
Graduate Program of Biotechnology, Federal University of Sao Carlos, 13565-905, São Carlos, SP, Brazil.
Appl Biochem Biotechnol. 2016 Jun;179(4):558-71. doi: 10.1007/s12010-016-2013-z. Epub 2016 Feb 18.
The use of residues from the industrial processing of palm oil as carbon source and inducer for microbial lipase production can be a way to add value to such residues and to contribute to reduced enzyme costs. The aim of this work was to investigate the feasibility of using palm oil industrial waste as feedstock for lipase production in different cultivation systems. Evaluation was made of lipase production by a selected strain of Aspergillus niger cultivated under solid-state (SSF) and submerged fermentation (SmF). Lipase activity levels up to 15.41 IU/mL were achieved under SSF. The effects of pH and temperature on the lipase activity of the SSF extract were evaluated using statistical design methodology, and maximum activities were obtained between pH 4.0 and 6.5 and at temperatures between 37 and 55 °C. This lipase presented good thermal stability up to 60 °C and higher specificity towards long carbon chain substrates. The results demonstrate the potential application of palm oil industrial residues for lipase production and contribute to the technological advances needed to develop processes for industrial enzymes production.
将棕榈油工业加工过程中的残留物用作微生物脂肪酶生产的碳源和诱导剂，可能是一种提高此类残留物附加值并降低酶成本的方法。本研究的目的是探讨在不同培养系统中使用棕榈油工业废料作为脂肪酶生产原料的可行性。对在固态发酵（SSF）和深层发酵（SmF）条件下培养的一株黑曲霉所产脂肪酶进行了评估。在固态发酵条件下，脂肪酶活性水平可达15.41 IU/mL。采用统计设计方法评估了pH值和温度对固态发酵提取物脂肪酶活性的影响，在pH 4.0至6.5以及温度37至55℃之间获得了最大活性。这种脂肪酶在高达60℃时具有良好的热稳定性，对长碳链底物具有更高的特异性。结果证明了棕榈油工业残留物在脂肪酶生产中的潜在应用，并有助于推动工业酶生产工艺所需的技术进步。
