Koch G L, Smith M J, Mortara R A
FEBS Lett. 1985 Jan 7;179(2):294-8. doi: 10.1016/0014-5793(85)80537-8.
Two-dimensional gel electrophoresis with the 125I-Con A overlay and affinity purification with Con A-agarose revealed the presence of an abundant ubiquitous 100-kDa glycoprotein (GP100) in nucleated mammalin cells. The amount in cultured human and murine cells varies from 3 to 20 X 10(6) molecules per cell making GP100 the most abundant glycoprotein in nucleated cells. Peptide mapping shows that it is different from erythrocyte Band III protein. Several properties of GP100 suggest that it could play a structural role in nucleated cell membranes.
采用125I-伴刀豆球蛋白A覆盖的二维凝胶电泳和伴刀豆球蛋白A-琼脂糖亲和纯化法，发现在有核哺乳动物细胞中存在一种丰富的普遍存在的100 kDa糖蛋白（GP100）。培养的人和鼠细胞中的含量为每个细胞3至20×10^6个分子，使GP100成为有核细胞中最丰富的糖蛋白。肽图谱分析表明它与红细胞带III蛋白不同。GP100的几个特性表明它可能在有核细胞膜中起结构作用。
