Nicollet I, Lebreton J P, Fontaine M, Hiron M
Biochim Biophys Acta. 1981 Apr 28;668(2):235-45. doi: 10.1016/0005-2795(81)90031-3.
Isoelectric focusing in polyacrylamide gel was used to reveal the two alpha 1-acid glycoprotein populations separated by affinity chromatography on Con A-Sepharose from human purified alpha 1-acid glycoprotein, whole normal serum and sera from patients undergoing an acute inflammatory process. The concanavalin A non-reactive peak had a lower isoelectric point than did the concanavalin A reactive peak. Moreover, crossed immunoaffinity electrophoresis with free concanavalin A revealed three alpha 1-acid glycoprotein populations (concanavalin A reactive, weakly and non-reactive). A significant difference was observed between the patterns of normal and of inflammatory sera, since the concanavalin A reactive fraction was enhanced during the inflammatory process. These results suggest a variation in relative ratios of the different types of peripheral oligosaccharide branches in the alpha 1-acid glycoprotein populations.
采用聚丙烯酰胺凝胶等电聚焦法，揭示了通过伴刀豆球蛋白A-琼脂糖亲和层析从人纯化α1-酸性糖蛋白、正常全血清及急性炎症患者血清中分离出的两个α1-酸性糖蛋白群体。伴刀豆球蛋白A无反应峰的等电点低于伴刀豆球蛋白A反应峰。此外，用游离伴刀豆球蛋白A进行交叉免疫亲和电泳显示出三个α1-酸性糖蛋白群体（伴刀豆球蛋白A反应性、弱反应性和无反应性）。正常血清和炎症血清的图谱之间存在显著差异，因为在炎症过程中伴刀豆球蛋白A反应性部分增加。这些结果表明α1-酸性糖蛋白群体中不同类型外周寡糖分支的相对比例存在差异。
