Koch G L, Smith M J
Eur J Biochem. 1982 Nov;128(1):107-12. doi: 10.1111/j.1432-1033.1982.tb06939.x.
The combination of two-dimensional gel electrophoresis and post-electrophoretic staining with 125I-labelled concanavalin A was used to compare the glycoproteins of murine tumour cell lines. Comparison between different cell lines showed that there were about eight common glycoproteins. The rest of the glycoproteins were generally unique to particular cells. Thus the P815 cell could be distinguished from 13 other murine cell lines by its glycoprotein pattern. The specific glycoproteins of each cell line were unaffected by culture in vivo, virus infection or hybridisation. Different clones from the same cell line gave identical patterns. Crude membrane preparations and glycoproteins purified from cell lysates by affinity chromatography on concanavalin/agarose gave the same patterns as whole cells. Thus the glycoproteins of murine tumour cells appear to be a stable characteristic which can provide specific markers for the identification of tumour cell lines.
二维凝胶电泳与用¹²⁵I标记的伴刀豆球蛋白A进行电泳后染色相结合，用于比较小鼠肿瘤细胞系的糖蛋白。不同细胞系之间的比较表明，约有八种常见糖蛋白。其余糖蛋白通常是特定细胞所特有的。因此，P815细胞可通过其糖蛋白模式与其他13种小鼠细胞系区分开来。每个细胞系的特异性糖蛋白不受体内培养、病毒感染或杂交的影响。来自同一细胞系的不同克隆呈现相同的模式。粗制膜制剂以及通过伴刀豆球蛋白/琼脂糖亲和层析从细胞裂解物中纯化的糖蛋白与完整细胞呈现相同的模式。因此，小鼠肿瘤细胞的糖蛋白似乎是一种稳定的特征，可为肿瘤细胞系的鉴定提供特异性标记。
