Liu Xinyi, Hong Dilong, Zhang Qian, Jiang Yuliang
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio- functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, Jiangsu, 210023, P.R. China.
J Fluoresc. 2024 Aug 24. doi: 10.1007/s10895-024-03908-1.
The rapid development of fluorescence probe technology has promoted in-depth research in fields such as environment and life medicine. Traditional single channel fluorescent probes can achieve highly sensitive detection of targets, but they appear powerless in complex environments. In addition, in today's deteriorating resource environment, implementing multi target detection with one probe can effectively save preparation resources, which is in line with the development direction of fluorescent probes. To achieve this goal, designing and preparing multi-site probes is undoubtedly the first choice, but the complexity of their preparation is daunting. Herein, we propose the concept of cascade detection for the first time. After the probe completes the first target detection, the complex between the probe and the first target is achieved based on the characteristics of the first target to achieve subsequent target detection. Based on this, a metal-organic framework was used as a basic skeleton and the concept of serial reactions was applied. First, copper ions were detected through coordination. Then, the specificity of copper for sulfur-containing amino acids was utilized to detect the three types of amino acids, and the practical applications of the three probes were studied separately.
荧光探针技术的快速发展推动了环境和生命医学等领域的深入研究。传统的单通道荧光探针能够实现对目标的高灵敏度检测，但在复杂环境中却显得无能为力。此外，在当今资源环境日益恶化的情况下，用一种探针实现多目标检测能够有效节省制备资源，这符合荧光探针的发展方向。为实现这一目标，设计和制备多位点探针无疑是首选，但它们制备过程的复杂性令人望而却步。在此，我们首次提出级联检测的概念。在探针完成第一次目标检测后，基于第一个目标的特性实现探针与第一个目标之间的复合物，以实现后续目标检测。基于此，以金属有机框架为基本骨架，并应用串联反应的概念。首先，通过配位检测铜离子。然后，利用铜对含硫氨基酸的特异性来检测这三种氨基酸，并分别研究了这三种探针的实际应用。
