The State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing, 210023, China.
Mikrochim Acta. 2024 Aug 24;191(9):561. doi: 10.1007/s00604-024-06629-1.
A novel "turn-on" aptasensor for kanamycin (Kana) detection based on a new Förster resonance energy transfer (FRET) pair is reported. A new organic small molecule was employed as a high-efficiency quencher for fluorophore. Based on specific interactions between ssDNA and the quencher, an ingenious and amplified strategy was designed. In the absence of the target, the fluorescence of the fluorophore labeled at the end of the aptamer was quenched. After the binding of the aptamer to the target, the fluorescence was recovered and amplified. The proposed aptasensor showed high specificity, selectivity, and stability in complicated systems. With the P3-based strategy, the limit of detection for Kana is estimated to be 10 nM, which is much lower than the maximum allowable concentration in milk. The recoveries of spiked Kana in milk were in the range 99.8 ~ 105.3% (n = 3). Fortunately, this novel method can be easily extended to other antibiotics such as tobramycin by simply replacing the aptamer, showing great potential as a universal platform for selective, sensitive, and rapid detection of hazardous analytes in food samples.
本文报道了一种基于新的Förster 共振能量转移（FRET）对的新型“开启”适体传感器，用于检测卡那霉素（Kana）。一种新型有机小分子被用作荧光团的高效猝灭剂。基于 ssDNA 与猝灭剂之间的特异性相互作用，设计了一种巧妙且放大的策略。在不存在靶标时，标记在适体末端的荧光团的荧光被猝灭。在适体与靶标结合后，荧光得到恢复和放大。所提出的适体传感器在复杂体系中表现出高特异性、选择性和稳定性。基于 P3 策略，Kana 的检测限估计为 10 nM，远低于牛奶中的最大允许浓度。牛奶中添加 Kana 的回收率在 99.8%~105.3%（n=3）范围内。幸运的是，通过简单地替换适体，这种新方法可以很容易地扩展到其他抗生素，如妥布霉素，显示出作为一种通用平台，用于选择性、灵敏和快速检测食品样品中危险分析物的巨大潜力。
