Wang Minji, Da Yifan, Tian Yang
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, No. 3663 Zhong Shan Road North, Shanghai, 200062, China.
Chem Soc Rev. 2023 Feb 20;52(4):1189-1214. doi: 10.1039/d2cs00419d.
The genetically encoded fluorescent sensors convert chemical and physical signals into light. They are powerful tools for the visualisation of physiological processes in living cells and freely moving animals. The fluorescent protein is the reporter module of a genetically encoded biosensor. In this study, we first review the history of the fluorescent protein in full emission spectra on a structural basis. Then, we discuss the design of the genetically encoded biosensor. Finally, we briefly review several major types of genetically encoded biosensors that are currently widely used based on their design and molecular targets, which may be useful for the future design of fluorescent biosensors.
基因编码荧光传感器可将化学和物理信号转化为光信号。它们是用于可视化活细胞和自由活动动物体内生理过程的强大工具。荧光蛋白是基因编码生物传感器的报告模块。在本研究中，我们首先在结构基础上全面回顾荧光蛋白全发射光谱的历史。然后，我们讨论基因编码生物传感器的设计。最后，我们根据其设计和分子靶点简要回顾目前广泛使用的几种主要类型的基因编码生物传感器，这可能对未来荧光生物传感器的设计有用。
