FrieslandCampina, Stationsplein 4, 3818 LE, Amersfoort, the Netherlands.
DUCARES B.V., Trading as TRISKELION, Reactorweg 47-A, 3542 AD, Utrecht, the Netherlands.
J AOAC Int. 2024 Nov 1;107(6):971-978. doi: 10.1093/jaoacint/qsae068.
Taurine (2-aminoethanesulfonic acid) is an essential β-amino acid, which is one of the most abundant intracellular amino acid component in humans. The level of free taurine in human milk is higher than in bovine milk. Since taurine is considered important for the development of a newborn, fortification of infant nutrition with taurine is common. This publication describes a rapid method for the determination of taurine in infant formula and adult/pediatric nutritionals.
This publication demonstrates the suitability and applicability of the method to determine taurine content in infant formula and adult/pediatric nutritionals.
Test portions are deproteinized by acid in an aqueous environment prior to analysis. The level of taurine is analyzed by hydrophilic interaction chromatography (HILIC) coupled with multiple reaction monitoring MS (MRM-MS). Quantitation is accomplished using a stable isotope-labeled internal standard (SILS) for taurine.
The accuracy of the method was validated using standard reference materials and spike recovery studies. The average recoveries ranged between 92% and 105%. The relative standard deviation repeatability (RSDr) and relative standard deviation intermediate precision (RSDiR) were in the range of 0.7-4.6% (RSDr) and 1.3-6.7% (RSDiR). The limit of quantitation (LOQ) was below the requirement of 0.5 mg/100 g.
The single-laboratory validation (SLV) of the method for determination of taurine content in infant formula and adult/pediatric nutritionals demonstrates that it meets the requirements per AOAC INTERNATIONAL Standard Method Performance Requirements (SMPR) 2014.013.
A fast method to selectively determine the content of taurine in infant formula and adult/pediatric nutritionals was evaluated and found to be fit for purpose for routine product compliance testing. Based on the results of this SLV, the method was approved by the AOAC Stakeholder Program on Infant Formula and Adult Nutritionals (SPIFAN) Expert Review Panel for SPIFAN Nutrient Methods for First Action Official MethodsSM status.
牛磺酸（2-氨基乙磺酸）是一种必需的β-氨基酸，是人体内最丰富的细胞内氨基酸成分之一。人乳中的游离牛磺酸水平高于牛乳。由于牛磺酸被认为对新生儿的发育很重要，因此在婴儿营养中添加牛磺酸是很常见的。本出版物描述了一种快速测定婴儿配方奶粉和成人/儿科营养品中牛磺酸的方法。
本出版物证明了该方法测定婴儿配方奶粉和成人/儿科营养品中牛磺酸含量的适用性和适用性。
在分析前，在水性环境中通过酸使测试部分脱蛋白。通过亲水相互作用色谱（HILIC）与多反应监测 MS（MRM-MS）联用分析牛磺酸的水平。通过牛磺酸的稳定同位素标记内标（SILS）进行定量。
使用标准参考物质和加标回收率研究验证了方法的准确性。平均回收率在 92%至 105%之间。重复性（RSDr）和中间精密度（RSDiR）的相对标准偏差分别在 0.7-4.6%（RSDr）和 1.3-6.7%（RSDiR）范围内。定量限（LOQ）低于 0.5mg/100g 的要求。
该方法用于测定婴儿配方奶粉和成人/儿科营养品中牛磺酸含量的单实验室验证（SLV）表明，该方法符合 AOAC 国际标准方法性能要求（SMPR）2014.013 的要求。
评估了一种快速选择性测定婴儿配方奶粉和成人/儿科营养品中牛磺酸含量的方法，发现该方法适用于常规产品合规性测试。基于这项 SLV 的结果，该方法获得了 AOAC 婴儿配方和成人营养利益相关者计划（SPIFAN）专家审查小组的批准，具有 SPIFAN 营养素方法的首次行动官方方法 SM 地位。
