单核细胞增生李斯特菌磷壁酸的酶免疫测定
Enzyme immunoassay of teichoic acids from Listeria monocytogenes.
作者信息
Kamisango K, Nagaoka M, Fujii H, Azuma I
出版信息
J Clin Microbiol. 1985 Jan;21(1):135-7. doi: 10.1128/jcm.21.1.135-137.1985.
Abstract
Amino groups were introduced into Listeria monocytogenes teichoic acids by reductive amination, and the product was coupled to biotin. Teichoic acids were assayed by their binding to specific antibody adsorbed to a solid phase, followed by detection of the antigen-antibody complex by horseradish peroxidase-avidin. Less than 20 ng of teichoic acid was detectable.
摘要
通过还原胺化反应将氨基引入单核细胞增生李斯特菌的磷壁酸中，然后将产物与生物素偶联。通过磷壁酸与吸附在固相上的特异性抗体结合进行检测，随后用辣根过氧化物酶-抗生物素蛋白检测抗原-抗体复合物。可检测到的磷壁酸含量低于20纳克。
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