Ullmann W W, Cameron J A
J Bacteriol. 1969 May;98(2):486-93. doi: 10.1128/jb.98.2.486-493.1969.
The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, galactose, rhamnose, and glucosamine; and types IVa and IVb, glucose and galactose. Quantitative precipitin inhibition tests with purified monosaccharides indicated that the major antigenic determinant of types I and II is rhamnose. Precipitin reactions could not be detected with type III carbohydrate and homologous or heterologous antisera. The major determinants of types IVa and IVb were found to be galactose and glucose, respectively. As much as 87% inhibition of the quantitative precipitin test for types I and II was obtained with rhamnose, 72% for type IVa with galactose, and 72% for type IVb with glucose. The immunochemical basis for the antigenic specificity of L. monocytogenes types I, II, IVa, and IVb was further confirmed by using agar gel diffusion. Cross-reactions among the various type-specific carbohydrates and heterologous antisera were also studied. Type II carbohydrate was found to contain galactose and react with type IVa antisera. This reaction could be blocked by galactose. Type I carbohydrate did not contain galactose nor did it react with antiserum prepared from type IVa cells. Therefore, the somatic antigens of type I and type II L. monocytogenes, previously thought to be identical, appeared to differ. The dominant immuno-specific group in the cross-reaction between type IVb carbohydrate and type IVa antisera was found to be galactose. Type IVa absorbed antisera did not produce a significant cross-reaction with type IVb carbohydrate. The results obtained from this investigation indicate a lesser degree of antigenic relationship between type IVa and type IVb L. monocytogenes than was previously believed to exist.
采用免疫化学技术研究了单核细胞增生李斯特菌Ⅰ型、Ⅱ型、Ⅲ型、Ⅳa型和Ⅳb型的抗原特异性。从细胞壁中提取了各型具有免疫活性的碳水化合物，并进行了化学分析。Ⅰ型和Ⅱ型主要含有葡糖胺和鼠李糖；Ⅲ型含有半乳糖、鼠李糖和葡糖胺；Ⅳa型和Ⅳb型含有葡萄糖和半乳糖。用纯化的单糖进行定量沉淀抑制试验表明，Ⅰ型和Ⅱ型的主要抗原决定簇是鼠李糖。Ⅲ型碳水化合物与同源或异源抗血清未检测到沉淀反应。发现Ⅳa型和Ⅳb型的主要决定簇分别是半乳糖和葡萄糖。鼠李糖对Ⅰ型和Ⅱ型定量沉淀试验的抑制率高达87%，半乳糖对Ⅳa型的抑制率为72%，葡萄糖对Ⅳb型的抑制率为72%。利用琼脂凝胶扩散法进一步证实了单核细胞增生李斯特菌Ⅰ型、Ⅱ型、Ⅳa型和Ⅳb型抗原特异性的免疫化学基础。还研究了各型特异性碳水化合物与异源抗血清之间的交叉反应。发现Ⅱ型碳水化合物含有半乳糖，并与Ⅳa型抗血清发生反应。该反应可被半乳糖阻断。Ⅰ型碳水化合物不含半乳糖，也不与由Ⅳa型细胞制备的抗血清发生反应。因此，先前认为相同的单核细胞增生李斯特菌Ⅰ型和Ⅱ型的菌体抗原似乎存在差异。发现Ⅳb型碳水化合物与Ⅳa型抗血清交叉反应中的主要免疫特异性基团是半乳糖。Ⅳa型吸收抗血清与Ⅳb型碳水化合物未产生明显的交叉反应。本次调查获得的结果表明，Ⅳa型和Ⅳb型单核细胞增生李斯特菌之间的抗原关系程度低于先前认为的存在程度。
