Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Food Chem. 2024 Dec 15;461:140928. doi: 10.1016/j.foodchem.2024.140928. Epub 2024 Aug 20.
Hydrogen peroxide (HO) is associated with diseases and food safety. Thus, it is essential to achieve sensitive and efficient detection of HO. Herein, a ratiometric luminescence composite nanoprobe was designed for single particle imaging sensing of HO by combining NaYbF:Er@NaYbF:Tm@NaGdF:Yb upconversion nanoparticles (UCNPs) with cyanine dye IR-628. High brightness NaYbF:Er@NaYbF:Tm@NaGdF:Yb UCNPs with double-peak emission (665 and 812 nm) were synthesised. Cyanine dye IR-628 with an absorption peak at 628 nm was synthesised and served as a recognition unit. More importantly, on the basis of the upconversion luminescence total internal reflection imaging technique, we developed a ratiometric single particle imaging quantitative analysis for sensing HO with a limit of quantitation of 5 nM. This ratiometric single particle imaging method not only greatly eliminates the influence of the probe concentration and instrumental and environmental factors, but also reduces the dosage of the reagent used and improves the sensitivity of detection.
过氧化氢（HO）与疾病和食品安全有关。因此，实现对 HO 的敏感和高效检测至关重要。在此，通过将上转换纳米粒子（UCNPs）NaYbF:Er@NaYbF:Tm@NaGdF:Yb 与菁染料 IR-628 结合，设计了一种比率型荧光复合纳米探针，用于 HO 的单颗粒成像传感。合成了具有双峰发射（665 和 812nm）的高亮度 NaYbF:Er@NaYbF:Tm@NaGdF:Yb UCNPs。合成了菁染料 IR-628，其吸收峰在 628nm 处，用作识别单元。更重要的是，基于上转换荧光全内反射成像技术，我们开发了一种比率型单颗粒成像定量分析方法，用于 HO 的传感，检出限为 5nM。这种比率型单颗粒成像方法不仅大大消除了探针浓度和仪器及环境因素的影响，而且减少了试剂的用量，提高了检测的灵敏度。
