Wiskocil R, Weiss A, Imboden J, Kamin-Lewis R, Stobo J
J Immunol. 1985 Mar;134(3):1599-603.
In the resting state, the T3-positive, human T cell line Jurkat does not synthesize detectable amounts of either interleukin 2 (IL 2) or gamma-interferon (IFN-gamma). Activation of Jurkat as measured by the secretion of substantial amounts of both lymphokines requires two distinct signals. One signal is produced by the phorbol ester, phorbol myristate acetate, and the other by either phytohemagglutinin or antibodies to T3. To elucidate the molecular events by which these activation signals lead to the synthesis of IL 2 and IFN-gamma activity we used cDNA probes to follow the appearance of IL 2 and IFN-gamma-specific transcripts after activation of Jurkat. These studies demonstrate that both signals are required for the appearance of IL 2 or IFN-gamma-specific transcripts and that the appearance of IL 2 and IFN-gamma RNA is coordinate with regard to a) the signals required for their production, b) the kinetics of their appearance, and c) the inhibition of their appearance by cyclosporin A. These studies suggest that distinct T cell-activation signals may operate through a common regulatory pathway involved in the expression of both IL 2 and IFN-gamma genes.
在静止状态下，T3 阳性的人 T 细胞系 Jurkat 不合成可检测量的白细胞介素 2（IL-2）或γ-干扰素（IFN-γ）。通过大量分泌这两种淋巴因子来衡量的 Jurkat 细胞激活需要两个不同的信号。一个信号由佛波酯、佛波醇肉豆蔻酸酯乙酸盐产生，另一个信号由植物血凝素或抗 T3 抗体产生。为了阐明这些激活信号导致 IL-2 和 IFN-γ活性合成的分子事件，我们使用 cDNA 探针追踪 Jurkat 细胞激活后 IL-2 和 IFN-γ特异性转录本的出现情况。这些研究表明，这两个信号都是 IL-2 或 IFN-γ特异性转录本出现所必需的，并且 IL-2 和 IFN-γ RNA 的出现在以下方面是协调一致的：a）它们产生所需的信号；b）它们出现的动力学；c）环孢菌素 A 对它们出现的抑制作用。这些研究表明，不同的 T 细胞激活信号可能通过参与 IL-2 和 IFN-γ基因表达的共同调节途径起作用。
