Sharkeev Yurii P, Komarova Ekaterina G, Chebodaeva Valentina V, Sedelnikova Mariya B, Zakharenko Aleksandr M, Golokhvast Kirill S, Litvinova Larisa S, Khaziakhmatova Olga G, Malashchenko Vladimir V, Yurova Kristina A, Gazatova Natalia D, Kozlov Ivan G, Khlusova Marina Y, Zaitsev Konstantin V, Khlusov Igor A
Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, Siberian Branch of Russian Academy of Sciences, 634055 Tomsk, Russia.
Research School of High-Energy Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
Materials (Basel). 2021 Jul 1;14(13):3693. doi: 10.3390/ma14133693.
A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous-crystalline structure that exhibits excellent biocopatibility. The structure and physico-chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO and β-CaPO were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from -456 to -535 mV, while the zeta potential (ZP) decreased from -53 to -40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200-250 V was strongly associated with elevated expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4 subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous-crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca as effectors of the biological effects of amorphous-crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.
创伤学、矫形外科学和植入学的一个现代趋势是开发具有非晶-晶体结构且表现出优异生物相容性的材料和涂层。研究了在不同电压（200、250和300V）下使用微弧氧化（MAO）方法沉积在钛板上的磷酸钙（CaP）涂层的结构、物理化学和生物学特性。使用透射电子显微镜（TEM）和X射线衍射（XRD）在涂层中观察到CaHPO和β-CaPO的非晶态、纳米晶态和微晶态。MAO电压的增加导致表面粗糙度从2.5增加到6.5µm，质量从10增加到25mg，厚度从50增加到105µm，Ca/P比从0.3增加到0.6。CaP涂层的电势（EP）从-456mV变为-535mV，而随着MAO电压值的增加，ζ电位（ZP）从-53mV降低到-40mV。估计了CaP涂层物理和化学指标的许多相关性。在200-250V下沉积的CaP涂层的ZP值降低与在用抗CD2/CD3/CD28抗体预先激活然后在体外与CaP涂层样品接触14天的肿瘤来源的Jurkat T细胞中表达升高密切相关。反过来，CD4亚群的体外存活率提高，同时激活的Jurkat T细胞分泌促炎细胞因子。因此，所施加的MAO电压在一定程度上允许调节钛基底上非晶-晶体CaP涂层的物理化学性质。该方法可作为一种技术机制，通过与微弧CaP涂层接触来触发细胞行为。讨论了负ZP和Ca作为非晶-晶体CaP涂层生物效应效应器的可能作用。微弧CaP涂层应仔细测试，以确定其在慢性淋巴细胞恶性肿瘤患者中的适用性。
