Schams D, Schallenberger E, Legros J J
J Reprod Fertil. 1985 Jan;73(1):165-71. doi: 10.1530/jrf.0.0730165.
In Exp. I, blood samples were collected simultaneously from the posterior vena cava and jugular vein or aorta from 7 heifers every 5-20 min for 2-5 h. Concomitant pulsatile secretion of oxytocin and immunoreactive neurophysin I was detected in the vena cava, but not in the jugular vein or aorta. Concentrations of oxytocin and immunoreactive neurophysin increased earlier and were higher in the vena cava than in the jugular vein or aorta after the injection of a luteolytic dose of prostaglandin F-2 alpha analogue during the mid-luteal phase of the oestrous cycle, demonstrating its ovarian but not pituitary origin. In Exp. II, blood samples were collected from the jugular vein every 12 h during 1 week after oestrus. Follicular growth had been stimulated during the preceding oestrous cycle with PMSG (10 heifers and cows) or with FSH (5 animals); 6 heifers served as controls. There was a high correlation between the number of follicles or CL and the increase in oxytocin and immunoreactive neurophysin I. Although PMSG had a greater luteotrophic effect than did FSH on progesterone secretion, a similar stimulation of oxytocin and immunoreactive neurophysin I was not observed. It is concluded that immunoreactive neurophysin I and oxytocin are secreted from the ovary in concentrations dependent upon the number of corpora lutea (and of follicles) present. During the mid-luteal period the secretion occurs in a concomitant pulsatile fashion.
在实验I中，从7头小母牛的后腔静脉和颈静脉或主动脉每隔5 - 20分钟同时采集血样，持续2 - 5小时。在后腔静脉中检测到催产素和免疫反应性神经垂体素I的伴随性脉冲分泌，但在颈静脉或主动脉中未检测到。在发情周期的黄体中期注射溶黄体剂量的前列腺素F-2α类似物后，后腔静脉中催产素和免疫反应性神经垂体素的浓度升高更早且更高，表明其来源于卵巢而非垂体。在实验II中，在发情后1周内每隔12小时从颈静脉采集血样。在前一个发情周期中，用孕马血清促性腺激素（PMSG，10头小母牛和母牛）或促卵泡素（FSH，5只动物）刺激卵泡生长；6头小母牛作为对照。卵泡或黄体的数量与催产素和免疫反应性神经垂体素I的增加之间存在高度相关性。虽然PMSG对孕酮分泌的黄体生成作用比FSH更强，但未观察到对催产素和免疫反应性神经垂体素I的类似刺激。结论是，免疫反应性神经垂体素I和催产素从卵巢分泌，其浓度取决于黄体（和卵泡）的数量。在黄体中期，分泌以伴随的脉冲方式发生。
