Vadas P, Stefanski E, Pruzanski W
Life Sci. 1985 Feb 11;36(6):579-87. doi: 10.1016/0024-3205(85)90640-x.
Phospholipase A2 (PLA2) activity has now been identified in rheumatoid synovial fluids. This PLA2 is a calcium-requiring protein of MW 11,000 with a neutral pH optimum. Its activity was inhibited by high concentrations of Mg2+, and by the active site-directed histidine reagent p-bromophenacyl bromide. Ionic and nonionic detergents, or the sulfhydryl reagent dithiothreitol caused loss of enzyme activity. Synovial fluid PLA2 did not interact with sulphated mucopolysaccharides such as heparin or chondroitin sulphate. Release and sequestration of PLA2 in the joint space may contribute to the characteristic rheumatoid inflammatory changes.
现已在类风湿性滑液中鉴定出磷脂酶A2（PLA2）活性。这种PLA2是一种分子量为11,000的需钙蛋白，最适pH值为中性。其活性受到高浓度Mg2+以及活性位点导向的组氨酸试剂对溴苯甲酰溴的抑制。离子和非离子去污剂或巯基试剂二硫苏糖醇会导致酶活性丧失。滑液PLA2不与硫酸化粘多糖如肝素或硫酸软骨素相互作用。PLA2在关节腔中的释放和隔离可能导致类风湿性特征性炎症变化。
