State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Key Laboratory of Yangtze River Water Environment, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Sci Total Environ. 2024 Nov 15;951:175758. doi: 10.1016/j.scitotenv.2024.175758. Epub 2024 Aug 23.
Organosulfur compounds are prevalent in wastewater, presenting challenges for biodegradation, particularly in low-carbon environments. Supplementing additional carbon sources not only provides essential nutrients for microbial growth but also serves as regulators, influencing adaptive changes in biofilm and enhancing the survival of microorganisms in organosulfur-induced stress bioreactors. This study aims to elucidate the biodegradation of organosulfur under varying carbon source levels, placing specific emphasis on functional bacteria and metabolic processes. It has been observed that higher levels of carbon supplementation led to significantly improved total sulfur (TS) removal efficiencies, exceeding 83 %, and achieve a high organosulfur CHSH removal efficiency of ~100 %. However, in the reactor with no external carbon source added, the oxidation end-product SO accumulated significantly, surpassing 120 mEq/m-day. Furthermore, the TB-EPS concentration consistently increasedwith the ascending glucose concentration. The analysis of bacterial community reveals the enrichment of functional bacteria involved in sulfur metabolism and biofilm formation (e.g. Ferruginibacter, Rhodopeudomonas, Gordonia, and Thiobacillus). Correspondingly, the gene expressions related to the pathway of organosulfur to SO were notably enhanced (e.g. MTO increased by 27.7 %). In contrast, extra carbon source facilitated the transfer of organosulfur into amino acids in sulfur metabolism and promoted assimilation. These metabolic insights, coupled with kinetic transformation results, further validate distinct sulfur pathways under different carbon source conditions. The intricate interplay between bacteria growth regulation, pollutant biodegradation, and microbial metabolites underscores a complex network relationship that significantly contributes to efficient operation of bioreactors.
有机硫化合物在废水中普遍存在，对生物降解构成挑战，特别是在低碳环境中。补充额外的碳源不仅为微生物生长提供了必需的营养物质，还作为调节剂，影响生物膜的适应性变化，并增强微生物在有机硫诱导的应激生物反应器中的生存能力。本研究旨在阐明不同碳源水平下有机硫的生物降解情况，特别关注功能细菌和代谢过程。研究结果表明，较高水平的碳源补充可显著提高总硫（TS）去除效率，超过 83%，并实现高达~100%的有机硫 CHSH 去除效率。然而，在未添加外部碳源的反应器中，氧化终产物 SO 显著积累，超过 120 mEq/m-day。此外，TB-EPS 浓度随着葡萄糖浓度的升高而持续增加。细菌群落分析揭示了参与硫代谢和生物膜形成的功能细菌的富集（如 Ferruginibacter、Rhodopeudomonas、Gordonia 和 Thiobacillus）。相应地，与有机硫到 SO 途径相关的基因表达显著增强（例如 MTO 增加了 27.7%）。相比之下，额外的碳源有利于有机硫在硫代谢中转化为氨基酸，并促进了同化。这些代谢见解与动力学转化结果相结合，进一步验证了不同碳源条件下的不同硫途径。细菌生长调节、污染物生物降解和微生物代谢物之间的复杂相互作用突显了一个复杂的网络关系，这对生物反应器的高效运行具有重要意义。
