Bouasla Nabila, Abderrahmane Sihem, Obeizi Zahra, Sarah Messast, Saoudi Adel
Université Chadli Bendjedid-El Tarf, B.P 73, El Taref, 36000, Algeria.
Surface Engineering Laboratory (L.I.S), Faculty of Sciences, Badji Mokhtar -Annaba University, P.o.Box 12, Annaba 23000, Algeria, Annaba, 23000, Algeria.
Chem Biodivers. 2024 Dec;21(12):e202400724. doi: 10.1002/cbdv.202400724. Epub 2024 Oct 22.
This study aims to synthesize ZnS nanoparticles (NPs) and investigate their biocidal effects, along with those of ZnO-Trioctylphosphine (ZnO-TOP) NPs, on various pathogenic microbes. The NPs were synthesized via the polyol method using the forced hydrolysis of zinc acetate. They were characterized by XRD and TEM. The average sizes of ZnS and ZnO-TOP are 3.63 nm and 16.28 nm, respectively. The antimicrobial activities were assessed using agar-well diffusion, minimum inhibitory concentration (MIC), and biofilm inhibition. The results showed that ZnS and ZnO-TOP NPs have potent antimicrobial activity against all tested pathogen microbes. A zone of maximum inhibition (ZMI) of 20±0.54 and 22±0.26 was observed in the case of ZnS for Acinetobacter baumannii and Candida albicans, respectively. For ZnO-TOP, a ZMI of 20±0.15 and 20±0.19 is obtained for Pseudomonas. aeruginosa ATCC 27853 and A. baumannii, respectively. Percentages of biofilm inhibition at 128 μg/ml were notably high for Enterococcus faecalis (96.83 % with ZnO-TOP and 91.17 % with ZnS) and Staphylococcus aureus (87.27 % with ZnO-TOP and 76.37 % with ZnS). The results suggest that ZnS and ZnO-TOP nanoparticles have promising potential as effective antimicrobial agents, especially against biofilm-forming pathogens, indicating their potential for future use in treating microbial infections.
本研究旨在合成硫化锌纳米颗粒（NPs），并研究其与氧化锌-三辛基膦（ZnO-TOP）纳米颗粒对各种致病微生物的杀菌作用。通过多元醇法利用醋酸锌的强制水解合成了这些纳米颗粒。它们通过X射线衍射（XRD）和透射电子显微镜（TEM）进行表征。硫化锌和ZnO-TOP的平均尺寸分别为3.63纳米和16.28纳米。使用琼脂孔扩散法、最低抑菌浓度（MIC）和生物膜抑制法评估抗菌活性。结果表明，硫化锌和ZnO-TOP纳米颗粒对所有测试的致病微生物都具有强大的抗菌活性。对于鲍曼不动杆菌和白色念珠菌，硫化锌的最大抑制圈（ZMI）分别为20±0.54和22±0.26。对于ZnO-TOP，铜绿假单胞菌ATCC 27853和鲍曼不动杆菌的ZMI分别为20±0.15和20±0.19。在128μg/ml时，粪肠球菌（ZnO-TOP为96.83%，硫化锌为91.17%）和金黄色葡萄球菌（ZnO-TOP为87.27%，硫化锌为76.37%）的生物膜抑制百分比显著较高。结果表明，硫化锌和ZnO-TOP纳米颗粒作为有效的抗菌剂具有广阔的前景，尤其是对形成生物膜的病原体，表明它们在未来治疗微生物感染方面具有潜在应用价值。
