利用 spp. 合成氧化锌纳米粒子及其抗菌效果研究。

Biosynthesis of Zinc Oxide Nanoparticles by spp. and Investigation of their Antimicrobial Effect.

机构信息

Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

出版信息

Curr Drug Discov Technol. 2023;20(5):e010523216388. doi: 10.2174/1570163820666230501152951.

DOI:10.2174/1570163820666230501152951

PMID:37138475

Abstract

BACKGROUND

Nanoparticle biology is preferable to other common methods due to its economic efficiency and compatibility with the environment. On the other hand, the prevalence of drug-resistant bacterial strains is expanding and it is necessary to use alternative antibiotic compounds to deal with them. The aim of the present study was the biosynthesis of zinc oxide nanoparticles(ZnO NPs) by Lactobacillus spp. and their antimicrobial effect.

METHODS

In this study, after the biosynthesis of ZnO NPs by Lactobacillus spp, Characterization of Nanoparticulation Was performed by UV-Vis, XRD, and Scanning Electron Microscopy (SEM). Additionally, Lactobacillus spp. - ZnO NPs were assessed for their antimicrobial properties.

RESULTS

UV-visible spectroscopy confirmed the Lactobacillus spp. - ZnO NPs absorbed UV in the region of 300-400 nm. XRD analysis showed the presence of zinc metal in nanoparticles. SEM revealed that Lactobacillus plantarum - ZnO NPs were smaller than the others. Staphylococcus aureus showed the largest non-growth halo diameter against ZnO NPs synthesized by L. plantarum ATCC 8014 (3.7 mm). E. coli had the largest growth halo diameter against ZnO NPs synthesized by L. casei (3 mm) and L. plantarum (2.9 mm). The MIC values of ZnO NPs synthesized by L. plantarum ATCC 8014, L.casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2,8,8 and 4 μg/mL for Staphylococcus aureus. The MIC values of ZnO NPs synthesized by L. plantarum ATCC 8014, L. casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2, 4, 4, and 4 μg/ml for E. coli. The lowest MICs were 2 μg/ml for E. coli and S. aureus related to ZnO NPs synthesized by L. plantarum ATCC 8014. MIC and MBC values were equivalent to each other.

CONCLUSION

The results of this research show that ZnO NPs synthesized by L. plantarum ATCC 8014 have more antimicrobial effects than other ZnO NPs used. Therefore, the ZnO NPs made with Lactobacillus plantarum ATCC 8014 have the potential to kill bacteria and can be considered a candidate for antibiotic replacement.

摘要

背景

由于其经济性和环境兼容性，纳米颗粒生物学优于其他常见方法。另一方面，耐药菌菌株的流行正在扩大，有必要使用替代抗生素化合物来应对它们。本研究的目的是通过 Lactobacillus spp 生物合成氧化锌纳米粒子（ZnO NPs）及其抗菌作用。

方法

在本研究中，Lactobacillus spp 生物合成 ZnO NPs 后，通过紫外-可见分光光度法、X 射线衍射（XRD）和扫描电子显微镜（SEM）对纳米粒子的形成进行了表征。此外，还评估了 Lactobacillus spp.-ZnO NPs 的抗菌性能。

结果

紫外-可见光谱证实 Lactobacillus spp.-ZnO NPs 在 300-400nm 区域吸收了紫外线。XRD 分析表明纳米颗粒中存在锌金属。SEM 显示 Lactobacillus plantarum-ZnO NPs 比其他 NPs 更小。金黄色葡萄球菌对由 L. plantarum ATCC 8014 合成的 ZnO NPs 表现出最大的非生长晕直径（3.7mm）。E. coli 对由 L. casei 合成的 ZnO NPs 表现出最大的生长晕直径（3mm）和 L. plantarum（2.9mm）。由 L. plantarum ATCC 8014、L.casei ATCC 39392、L. fermenyum ATCC 9338 和 L.acidophilus ATCC 4356 合成的 ZnO NPs 对金黄色葡萄球菌的 MIC 值分别为 2、8、8 和 4μg/ml。由 L. plantarum ATCC 8014、L. casei ATCC 39392、L. fermenyum ATCC 9338 和 L. acidophilus ATCC 4356 合成的 ZnO NPs 对大肠杆菌的 MIC 值分别为 2、4、4 和 4μg/ml。与由 L. plantarum ATCC 8014 合成的 ZnO NPs 相比，E.coli 和 S.aureus 相关的最低 MIC 值为 2μg/ml。MIC 和 MBC 值相等。