Dupont E, Huygen K, Schandené L, Vandercruys M, Palfliet K, Wybran J
Transplantation. 1985 Feb;39(2):143-7. doi: 10.1097/00007890-198502000-00007.
The influence in vivo of immunosuppressive drugs (cyclosporine, azathioprine, and corticosteroids) on the production of various lymphokines (alpha and gamma interferon, interleukin 2), both in organ transplant recipients and in normal volunteers taking 100 mg hydrocortisone orally has been studied. To avoid interference with the rejection process or viral infection, patients were studied in a steady state with low maintenance immunosuppression consisting of prednisolone combined with azathioprine or with cyclosporine. In patients treated with both drug regimens, significant depression of production of the three lymphokines was found. Normal volunteers challenged with 100 mg hydrocortisone showed inhibition of production of interleukin 2 and alpha and gamma interferon in 4 hr, a time corresponding to the nadir of T cell lymphopenia, affecting the OKT4 subset preferentially. The percentage of OKT8 cells remained unchanged. Percentages of large granular lymphocytes increased, but their absolute number was not significantly modified. Changes in lymphocyte markers were fully reversible after 24 hr, but interleukin 2 production remained markedly depressed, showing that the redistribution patterns induced by corticosteroids on lymphocyte subsets may be dissociated from functional consequences.
研究了免疫抑制药物（环孢素、硫唑嘌呤和皮质类固醇）在体内对器官移植受者以及口服100毫克氢化可的松的正常志愿者体内各种淋巴因子（α和γ干扰素、白细胞介素2）产生的影响。为避免干扰排斥反应过程或病毒感染，对处于稳定状态、采用由泼尼松龙联合硫唑嘌呤或环孢素组成的低维持剂量免疫抑制方案的患者进行了研究。在用两种药物方案治疗的患者中，发现三种淋巴因子的产生均显著降低。用100毫克氢化可的松刺激的正常志愿者在4小时时显示白细胞介素2以及α和γ干扰素的产生受到抑制，这一时刻与T细胞淋巴细胞减少的最低点相对应，且优先影响OKT4亚群。OKT8细胞的百分比保持不变。大颗粒淋巴细胞的百分比增加，但其绝对数量没有显著改变。淋巴细胞标志物的变化在24小时后完全可逆，但白细胞介素2的产生仍显著降低，这表明皮质类固醇对淋巴细胞亚群诱导的再分布模式可能与其功能后果相分离。
