Lin Yibin, Liu Lehua, Lu Siyang, Fan Linqi, Hu Huaqi, Wang Xuanyin, Zhu Jichao, Qiang Xinhua, He Jie, Zhou Hongchang, Shao Shengwen, Zheng Gaoming
School of Medicine, Huzhou University, Huzhou, 313000, People's Republic of China.
Key Laboratory for Precise Prevention and Control of Major Chronic Diseases, Huzhou University, Huzhou, 31300, People's Republic of China.
Infect Drug Resist. 2024 Aug 19;17:3623-3635. doi: 10.2147/IDR.S477637. eCollection 2024.
is a common bacterium found in the environment. Some strains can cause food poisoning, and very few can cause clinically severe infections, leading to death. Here, we characterized the genome sequence of LIN78 isolated from teeth with deep caries and compared it with those of 25 other related species.
Third-generation sequencing technology, bacteriological analyses, biochemistry, and mass spectrometry were applied to characterize the drug-resistance genes and virulence factors of LIN78.
The complete genome sequence of Lin78 consists of 5647 genes distributed on a circular chromosome, a 393 kbp plasmid, and 928 pseudogenes (37.4% of whole-genome DNA). The LIN78 genome contains 14 sets of 16s, 23s, and 5s ribosomal RNA operons; 106 tRNA genes, one tmRNA, 12 genomic islands, six prophases, 64 repeats; 37 antibiotic-resistant genes; and 1119 putative virulence genes, including enterotoxins and cytolysins. The LIN78 genome carries multiple copies of non-ribosomal polypeptide synthetase (NRPS) and post-translationally modified peptides (RiPPs). Phylogenetic analysis of the 26 strains showed that LIN78 is evolutionarily closely related to ATCC 10792 and ATCC 14579.
The newly isolated carries many virulence genes, including enterotoxins and hemolysins, similar to , and multiple antibiotic resistance genes. These findings suggest that the strain has a potential risk of causing disease. Our studies are vital for further exploration of the evolution of , its pathogenic mechanisms, and the control and treatment of bacterial infections.
是一种在环境中常见的细菌。一些菌株可导致食物中毒，极少数可引起临床严重感染并导致死亡。在此，我们对从深龋牙齿中分离出的LIN78的基因组序列进行了表征，并将其与其他25个相关物种的基因组序列进行了比较。
应用第三代测序技术、细菌学分析、生物化学和质谱来表征LIN78的耐药基因和毒力因子。
Lin78的完整基因组序列由分布在一条环状染色体、一个393 kbp质粒和928个假基因（占全基因组DNA的37.4%）上的5647个基因组成。LIN78基因组包含14套16S、23S和5S核糖体RNA操纵子；106个tRNA基因、一个tmRNA、12个基因组岛、6个前噬菌体、64个重复序列；37个抗生素抗性基因；以及1119个推定的毒力基因，包括肠毒素和细胞溶素。LIN78基因组携带多个非核糖体多肽合成酶（NRPS）和翻译后修饰肽（RiPPs）的拷贝。对这26个菌株的系统发育分析表明，LIN78在进化上与ATCC 10792和ATCC 14579密切相关。
新分离的菌株携带许多毒力基因，包括与……相似的肠毒素和溶血素，以及多个抗生素抗性基因。这些发现表明该菌株具有致病的潜在风险。我们的研究对于进一步探索……的进化、其致病机制以及细菌感染的控制和治疗至关重要。
