Chen Jiancai, Zhang Junyan, Zhan Li, Chen Honghu, Zhang Zhen, Huang Cheng, Yue Min
Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Hainan Institute, Zhejiang University, Hangzhou, China.
Front Microbiol. 2022 Jul 15;13:964823. doi: 10.3389/fmicb.2022.964823. eCollection 2022.
is a major food-borne bacterial pathogen in the world, which can cause diarrhea and emetic syndrome. This study aimed to reveal the quantitative prevalence of in ready-to-eat (RTE) rice products in Eastern China and to gain essential information on the characteristics of isolates. A total of 91 out of the 1071 samples were positive for . The contamination level of in 0.5 % of RTE rice product samples outnumbered 10 CFU/g. The number of attained 10-10 CFU/g in one sample. The distribution patterns of virulence genes in isolates were identified. 84.6% of the isolates had at least one enterotoxin or emetic toxin gene. The predominant pattern was XXV. 9.9% of isolates belonged to it and possessed one enterotoxin gene . The occurrence rate of and was 36.3% and 47.3%, respectively. Antimicrobial susceptibility tests revealed a high resistance rate toward penicillin, and 23.1% of the isolates were multi-drug resistant. isolates were genotyped by using ERIC-PCR. 89 genotypes were determined. The Hunter Gaston Discriminatory Index (HGDI) attained 0.9995. Relationships analysis revealed that Group A isolates tended to carry , , , , and show resistance to penicillin/trimethoprim/sulfamethoxazole. This study was useful for updating the knowledge of the contamination status of in RTE rice products in China.
是世界上一种主要的食源性细菌病原体，可引起腹泻和呕吐综合征。本研究旨在揭示华东地区即食（RTE）大米制品中该菌的定量流行情况，并获取该菌分离株特征的基本信息。1071份样本中共有91份该菌检测呈阳性。0.5%的即食大米制品样本中该菌的污染水平超过10 CFU/g。有一个样本中该菌数量达到10⁵ - 10⁶ CFU/g。鉴定了该菌分离株中毒力基因的分布模式。84.6%的该菌分离株至少有一个肠毒素或呕吐毒素基因。主要模式为XXV。9.9%的分离株属于该模式且拥有一个肠毒素基因。该菌和该菌的发生率分别为36.3%和47.3%。药敏试验显示对青霉素的耐药率很高，23.1%的分离株为多重耐药。使用ERIC-PCR对该菌分离株进行基因分型。确定了89种基因型。亨特·加斯顿鉴别指数（HGDI）达到0.9995。相关性分析表明，A组该菌分离株倾向于携带该菌、该菌、该菌、该菌，并对青霉素/甲氧苄啶/磺胺甲恶唑耐药。本研究有助于更新中国即食大米制品中该菌污染状况的知识。
