Ramirez-Castillo Rosa, Palma-Rojas Claudio, Seguel Pedro Jara, Grusz Amanda L, Araya-Jaime Cristian
Departamento de Biología Universidad de La Serena La Serena Chile.
Núcleo de Estudios Ambientales, Facultad de Recursos Naturales Universidad Católica de Temuco Temuco Chile.
Appl Plant Sci. 2024 Jun 4;12(4):e11588. doi: 10.1002/aps3.11588. eCollection 2024 Jul-Aug.
Cytotaxonomy employs chromosome visualization to study organismal relationships and evolution. Despite the critical value of cytogenetic data, cytotypes are lacking for many plant groups. Here, we present an improved approach for visualizing mitotic chromosomes in ferns, a key lineage of land plants, using the dividing cells of unfurling croziers (fiddleheads).
Our modified mitotic chromosome preparation incorporates a brief pectinase-cellulase pretreatment, as well as colchicine fixation and the Feulgen reaction to improve the staining and separation of mitotic chromosomes. To demonstrate this easy and efficient assessment, we determined the sporophytic (2) chromosome number for three fern species: (2 = 60), (2 = 120), and (2 = 82)
The new method presented here improves visualizations of mitotic chromosomes from the dividing nuclei of young fern croziers. Fiddleheads are widely accessible in nature and in living collections worldwide, and this modified approach increases their suitability for fern cytotaxonomic studies.
细胞分类学利用染色体可视化来研究生物间的关系和进化。尽管细胞遗传学数据具有重要价值，但许多植物类群仍缺乏细胞型。在此，我们提出一种改进方法，利用展开的拳卷幼叶（嫩蕨）的分裂细胞来可视化蕨类植物（陆地植物的一个关键谱系）的有丝分裂染色体。
我们改良的有丝分裂染色体制备方法包括短暂的果胶酶 - 纤维素酶预处理，以及秋水仙素固定和福尔根反应，以改善有丝分裂染色体的染色和分离效果。为证明这种简便有效的评估方法，我们确定了三种蕨类植物的孢子体（2n）染色体数目：（2n = 60）、（2n = 120）和（2n = 82）。
本文提出的新方法改进了对幼嫩蕨类拳卷幼叶分裂细胞核中有丝分裂染色体的可视化效果。拳卷幼叶在自然界和世界各地的活体收藏中广泛可得，这种改良方法提高了它们在蕨类细胞分类学研究中的适用性。
