Yang Nai-Ying, Jia Xin-Lei, Sui Chang-Xu, Shen Shi-Yi, Dai Xi-Ling, Xue Jing-Shi, Yang Zhong-Nan
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Front Plant Sci. 2022 Apr 29;13:878693. doi: 10.3389/fpls.2022.878693. eCollection 2022.
Reconstructing the development of sporangia in seed-free vascular plants provides crucial information about key processes enabling the production of spores that are important in the life cycle of these plants. By applying fluorescence imaging in intact tissues using dyes and confocal microscopy, this study aimed to reconstruct the key steps during the development of sporangia. Special emphasis was taken on the cell wall structures of tapetum and spore mother cells that have been challenged by microscopical documentation in the past. After staining the cell wall and cytoplasm using calcofluor white and basic fuchsin, the sporangium development of was observed using confocal microscopy. The clear cell lineages from the sporangial initial cell to stalk, epidermis, inner tapetum, outer tapetum, and sporogenous cells were revealed by confocal imaging. The sporangium development improved in this work will be useful for a general understanding of fern spore formation.
重建无种子维管植物中孢子囊的发育过程，可为这些植物生命周期中产生重要孢子的关键过程提供关键信息。通过使用染料和共聚焦显微镜对完整组织进行荧光成像，本研究旨在重建孢子囊发育过程中的关键步骤。过去，绒毡层和孢子母细胞的细胞壁结构一直是显微镜记录的难题，本次研究对此给予了特别关注。使用荧光增白剂和碱性品红对细胞壁和细胞质进行染色后，利用共聚焦显微镜观察了孢子囊的发育过程。共聚焦成像揭示了从孢子囊原始细胞到柄、表皮、内层绒毡层、外层绒毡层和造孢细胞的清晰细胞谱系。本研究中改进的孢子囊发育研究方法，将有助于人们对蕨类植物孢子形成有更全面的了解。
