Shankar Nishanth R, Garnica Lorena, Stahlschmidt Emily, Byers Derek, Kulkarni Hrishikesh
Kasturba Medical College, Mangalore, India 575001.
Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, MO 63110.
bioRxiv. 2024 Aug 12:2024.08.10.607431. doi: 10.1101/2024.08.10.607431.
Many fundamental discoveries have occurred using primary cells from deceased donor lungs. These cells respond differently to injury when there are underlying co-morbidities like diabetes mellitus, hypertension, aging and exposures to cigarette smoke, cocaine and chronic alcohol use. However, the prevalence of these characteristics in donor lungs utilized for research is currently unknown.
This retrospective cohort study procured data of lung transplant donors from Mid-America Transplant from January 2017 until July 2023. The donors were characterized based on lung utilization into three groups - lungs used for research, lungs used for transplant, and lungs not recovered from donors for either research or transplantation.
The mean age of donors whose lungs were utilized for research was 41±18 years. 25% of them were expanded criteria donors (ECD) while 10% of the donors in the transplant cohort were ECD. 14% of the donors whose lungs were utilized for research had history of diabetes compared to 8% of donors whose lungs were transplanted. A quarter of the research donor population had positive history of cigarette use within the preceding 20 years. At least 40% of donors had a positive history of non-intravenous drug use, of whom a majority had a history of continued non-intravenous drug use.
No strict selection criteria or protocols exist when human donor lungs are obtained for ex-vivo research. There is a high prevalence of diabetes mellitus, history of smoking and non-intravenous drug use along with older age distribution in donors whose lungs used are for research.
许多重要的发现都是通过使用已故供体肺的原代细胞取得的。当存在诸如糖尿病、高血压、衰老以及接触香烟烟雾、可卡因和长期酗酒等潜在合并症时，这些细胞对损伤的反应会有所不同。然而，目前尚不清楚用于研究的供体肺中这些特征的普遍程度。
这项回顾性队列研究收集了2017年1月至2023年7月期间来自美国中部移植中心的肺移植供体数据。根据肺的用途将供体分为三组——用于研究的肺、用于移植的肺以及未从供体获取用于研究或移植的肺。
其肺用于研究的供体的平均年龄为41±18岁。其中25%为扩大标准供体（ECD），而移植队列中的供体有10%为ECD。其肺用于研究的供体中有14%有糖尿病史，而肺被移植的供体中有8%有糖尿病史。四分之一的研究供体人群在过去20年内有吸烟史。至少40%的供体有非静脉吸毒史，其中大多数有持续非静脉吸毒史。
获取人类供体肺用于体外研究时，不存在严格的选择标准或方案。其肺用于研究的供体中糖尿病、吸烟史和非静脉吸毒的患病率较高，且年龄分布较大。
