• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

靶向关键脂蛋白受体的毛菊苣苷脂质体对小鼠帕金森病模型有效。

Key Lipoprotein Receptor Targeted Echinacoside-Liposomes Effective Against Parkinson's Disease in Mice Model.

机构信息

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.

出版信息

Int J Nanomedicine. 2024 Aug 19;19:8463-8483. doi: 10.2147/IJN.S468942. eCollection 2024.

DOI:10.2147/IJN.S468942
PMID:39185346
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11342948/
Abstract

INTRODUCTION

Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the degeneration of dopaminergic neurons in the substantia nigra. The precise molecular mechanisms underlying neuronal loss in PD remain unknown, and there are currently no effective treatments for PD-associated neurodegeneration. Echinacoside (ECH) is known for its neuroprotective effects, which include scavenging cellular reactive oxygen species and promoting mitochondrial fusion. However, the blood-brain barrier (BBB) limits the bioavailability of ECH in the brain, posing a significant challenge to its use in PD treatment.

METHODS

We synthesized and characterized PEGylated ECH liposomes (ECH@Lip) and peptide angiopep-2 (ANG) modified liposomes (ECH@ANG-Lip). The density of ANG in ANG-Lip was optimized using bEnd.3 cells. The brain-targeting ability of the liposomes was assessed in vitro using a transwell BBB model and in vivo using an imaging system and LC-MS. We evaluated the enhanced neuroprotective properties of this formulation in a the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD model.

RESULTS

The ECH@ANG-Lip demonstrated significantly higher whole-brain uptake compared to ECH@Lip and free ECH. Furthermore, ECH@ANG-Lip was more effective in mitigating MPTP-induced behavioral impairment, oxidative stress, dopamine depletion, and dopaminergic neuron death than both ECH@Lip and free ECH.

CONCLUSION

The formulation used in our study significantly enhanced the neuroprotective efficacy of ECH in the MPTP-induced PD model. Thus, ECH@ANG-Lip shows considerable potential for improving the bioavailability of ECH and providing neuroprotective effects in the brain.

摘要

简介

帕金森病（PD）是一种常见的神经退行性疾病，其特征是黑质中多巴胺能神经元的退化。PD 中神经元丧失的确切分子机制尚不清楚，目前也没有有效的 PD 相关神经退行性病变治疗方法。松果菊苷（ECH）以其神经保护作用而闻名，包括清除细胞活性氧和促进线粒体融合。然而，血脑屏障（BBB）限制了 ECH 在大脑中的生物利用度，这对其在 PD 治疗中的应用构成了重大挑战。

方法

我们合成并表征了聚乙二醇化 ECH 脂质体（ECH@Lip）和肽血管生成素-2（ANG）修饰的脂质体（ECH@ANG-Lip）。使用 bEnd.3 细胞优化了 ANG-Lip 中的 ANG 密度。通过体外转染 BBB 模型和体内成像系统和 LC-MS 评估了脂质体的脑靶向能力。我们在 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的 PD 模型中评估了这种配方的增强神经保护特性。

结果

ECH@ANG-Lip 与 ECH@Lip 和游离 ECH 相比，表现出明显更高的全脑摄取。此外，ECH@ANG-Lip 比 ECH@Lip 和游离 ECH 更能减轻 MPTP 诱导的行为障碍、氧化应激、多巴胺耗竭和多巴胺能神经元死亡。

结论

我们研究中使用的配方显著增强了 ECH 在 MPTP 诱导的 PD 模型中的神经保护作用。因此，ECH@ANG-Lip 显示出在提高 ECH 的生物利用度和提供脑内神经保护作用方面的巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/9045edd7ff3b/IJN-19-8463-g0012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/5f3b6b971a98/IJN-19-8463-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/f89da3d31c63/IJN-19-8463-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/86bb0f35f22c/IJN-19-8463-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/940c71d51b0a/IJN-19-8463-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/ada859b4547b/IJN-19-8463-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/de72280b06f3/IJN-19-8463-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/f7767a82589c/IJN-19-8463-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/8ea8c88c25cc/IJN-19-8463-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/dfb32f59bd9c/IJN-19-8463-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/ddbf8e4f5d9e/IJN-19-8463-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/42518fecfbe8/IJN-19-8463-g0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/9045edd7ff3b/IJN-19-8463-g0012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/5f3b6b971a98/IJN-19-8463-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/f89da3d31c63/IJN-19-8463-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/86bb0f35f22c/IJN-19-8463-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/940c71d51b0a/IJN-19-8463-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/ada859b4547b/IJN-19-8463-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/de72280b06f3/IJN-19-8463-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/f7767a82589c/IJN-19-8463-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/8ea8c88c25cc/IJN-19-8463-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/dfb32f59bd9c/IJN-19-8463-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/ddbf8e4f5d9e/IJN-19-8463-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/42518fecfbe8/IJN-19-8463-g0011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d7b/11342948/9045edd7ff3b/IJN-19-8463-g0012.jpg

相似文献

1
Key Lipoprotein Receptor Targeted Echinacoside-Liposomes Effective Against Parkinson's Disease in Mice Model.靶向关键脂蛋白受体的毛菊苣苷脂质体对小鼠帕金森病模型有效。
Int J Nanomedicine. 2024 Aug 19;19:8463-8483. doi: 10.2147/IJN.S468942. eCollection 2024.
2
Echinacoside protects dopaminergic neurons by inhibiting NLRP3/Caspase-1/IL-1β signaling pathway in MPTP-induced Parkinson's disease model.松果菊苷通过抑制 MPTP 诱导的帕金森病模型中的 NLRP3/Caspase-1/IL-1β 信号通路保护多巴胺能神经元。
Brain Res Bull. 2020 Nov;164:55-64. doi: 10.1016/j.brainresbull.2020.08.015. Epub 2020 Aug 23.
3
Neuroprotective Effect of Echinacoside in Subacute Mouse Model of Parkinson's Disease.松果菊苷对帕金森病亚急性模型小鼠的神经保护作用。
Biomed Res Int. 2019 Jan 30;2019:4379639. doi: 10.1155/2019/4379639. eCollection 2019.
4
The intranasal administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP): a new rodent model to test palliative and neuroprotective agents for Parkinson's disease.鼻腔内给予 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）：一种用于测试帕金森病姑息治疗和神经保护剂的新啮齿动物模型。
Curr Pharm Des. 2011;17(5):489-507. doi: 10.2174/138161211795164095.
5
Systemically administered neuregulin-1β1 rescues nigral dopaminergic neurons via the ErbB4 receptor tyrosine kinase in MPTP mouse models of Parkinson's disease.在帕金森病的MPTP小鼠模型中，全身给药的神经调节蛋白-1β1通过ErbB4受体酪氨酸激酶挽救黑质多巴胺能神经元。
J Neurochem. 2015 May;133(4):590-7. doi: 10.1111/jnc.13026. Epub 2015 Jan 26.
6
Neuroprotective efficacy of a new brain-penetrating C-Abl inhibitor in a murine Parkinson's disease model.新型穿透血脑屏障的 C-Abl 抑制剂在帕金森病小鼠模型中的神经保护作用。
PLoS One. 2013 May 31;8(5):e65129. doi: 10.1371/journal.pone.0065129. Print 2013.
7
Neuroprotective effect of silymarin in a MPTP mouse model of Parkinson's disease.水飞蓟素在帕金森病MPTP小鼠模型中的神经保护作用。
Toxicology. 2014 May 7;319:38-43. doi: 10.1016/j.tox.2014.02.009. Epub 2014 Mar 4.
8
Transferrin-Modified Osthole PEGylated Liposomes Travel the Blood-Brain Barrier and Mitigate Alzheimer's Disease-Related Pathology in APP/PS-1 Mice.转铁蛋白修饰的蛇床子素聚乙二醇脂质体通过血脑屏障并减轻 APP/PS-1 小鼠的阿尔茨海默病相关病理。
Int J Nanomedicine. 2020 Apr 23;15:2841-2858. doi: 10.2147/IJN.S239608. eCollection 2020.
9
Neuroprotective Effects of Echinacoside on Regulating the Stress-Active p38MAPK and NF-κB p52 Signals in the Mice Model of Parkinson's Disease.紫锥菊苷对帕金森病小鼠模型中应激激活的p38丝裂原活化蛋白激酶和核因子κB p52信号的神经保护作用
Neurochem Res. 2017 Apr;42(4):975-985. doi: 10.1007/s11064-016-2130-7. Epub 2016 Dec 15.
10
Anti-apoptotic effect of modified Chunsimyeolda-tang, a traditional Korean herbal formula, on MPTP-induced neuronal cell death in a Parkinson's disease mouse model.韩国传统草药配方改良春四妙汤对帕金森病小鼠模型中MPTP诱导的神经元细胞死亡的抗凋亡作用
J Ethnopharmacol. 2015 Dec 24;176:336-44. doi: 10.1016/j.jep.2015.11.013. Epub 2015 Nov 23.

引用本文的文献

1
Crossing the blood-brain barrier: nanoparticle-based strategies for neurodegenerative disease therapy.穿越血脑屏障：基于纳米颗粒的神经退行性疾病治疗策略
Drug Deliv Transl Res. 2025 Jun 14. doi: 10.1007/s13346-025-01887-9.
2
Vesicular Carriers for Phytochemical Delivery: A Comprehensive Review of Techniques and Applications.用于植物化学物质递送的囊泡载体：技术与应用综述
Pharmaceutics. 2025 Apr 2;17(4):464. doi: 10.3390/pharmaceutics17040464.

本文引用的文献

1
Strategies for non-viral vectors targeting organs beyond the liver.靶向肝脏以外器官的非病毒载体策略。
Nat Nanotechnol. 2024 Apr;19(4):428-447. doi: 10.1038/s41565-023-01563-4. Epub 2023 Dec 27.
2
Fusogenic liposomes encapsulating mitochondria as a promising delivery system for osteoarthritis therapy.融合脂质体包裹线粒体作为骨关节炎治疗的有前途的递药系统。
Biomaterials. 2023 Nov;302:122350. doi: 10.1016/j.biomaterials.2023.122350. Epub 2023 Oct 15.
3
[Regulative effect of active components of Cistanche deserticola on intestinal dysbacteriosis induced by antibiotics in mice].
[肉苁蓉活性成分对小鼠抗生素诱导的肠道菌群失调的调节作用]
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Nov;38(6):766-770. doi: 10.12047/j.cjap.6381.2022.139.
4
Allomelanin-based biomimetic nanotherapeutics for orthotopic glioblastoma targeted photothermal immunotherapy.基于黑素体的仿生纳米疗法用于胶质母细胞瘤的原位光热免疫治疗。
Acta Biomater. 2023 Aug;166:552-566. doi: 10.1016/j.actbio.2023.05.037. Epub 2023 May 24.
5
Interpreting the Therapeutic Efficiency of Multifunctional Hybrid Nanostructure against Glioblastoma.解读多功能杂化纳米结构对胶质母细胞瘤的治疗效果
ACS Omega. 2023 Mar 22;8(13):12259-12267. doi: 10.1021/acsomega.2c08265. eCollection 2023 Apr 4.
6
Impaired dopamine release in Parkinson's disease.帕金森病中多巴胺释放的受损。
Brain. 2023 Aug 1;146(8):3117-3132. doi: 10.1093/brain/awad064.
7
Liposome-based diagnostic and therapeutic applications for pancreatic cancer.基于脂质体的胰腺癌诊断与治疗应用。
Acta Biomater. 2023 Feb;157:1-23. doi: 10.1016/j.actbio.2022.12.013. Epub 2022 Dec 12.
8
LDL receptor-related protein 1 (LRP1), a novel target for opening the blood-labyrinth barrier (BLB).低密度脂蛋白受体相关蛋白 1（LRP1），血迷路屏障（BLB）开放的新靶点。
Signal Transduct Target Ther. 2022 Jun 10;7(1):175. doi: 10.1038/s41392-022-00995-z.
9
Enhancement of blood-brain barrier penetration and the neuroprotective effect of resveratrol.增强血脑屏障通透性和白藜芦醇的神经保护作用。
J Control Release. 2022 Jun;346:1-19. doi: 10.1016/j.jconrel.2022.04.003. Epub 2022 Apr 6.
10
Claudin-5 binder enhances focused ultrasound-mediated opening in an blood-brain barrier model.Claudin-5 结合剂增强了血脑屏障模型中的聚焦超声介导的开放。
Theranostics. 2022 Jan 31;12(5):1952-1970. doi: 10.7150/thno.65539. eCollection 2022.