College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P. R. China.
Biomacromolecules. 2024 Sep 9;25(9):5609-5629. doi: 10.1021/acs.biomac.4c00795. Epub 2024 Aug 26.
Self-assembly, a powerful strategy for constructing highly stable and well-ordered supramolecular structures, widely exists in nature and in living systems. Peptides are frequently used as building blocks in the self-assembly process due to their advantageous characteristics, such as ease of synthesis, tunable mechanical stability, good biosafety, and biodegradability. Among the initiators for peptide self-assembly, enzymes are excellent candidates for guiding this process under mild reaction conditions. As a crucial and commonly used biomarker, alkaline phosphatase (ALP) cleaves phosphate groups, triggering a hydrophilicity-to-hydrophobicity transformation that induces peptide self-assembly. In recent years, ALP-instructed peptide self-assembly has made breakthroughs in biological imaging and therapy, inspiring the development of self-assembly biomaterials for diagnosis and therapeutics. In this review, we highlight the most recent advancements in ALP-instructed peptide assemblies and provide perspectives on their potential impact. Finally, we briefly discuss the ongoing challenges for future research in this field.
自组装是一种构建高度稳定和有序的超分子结构的强大策略，广泛存在于自然界和生命系统中。由于其具有易于合成、可调节的机械稳定性、良好的生物安全性和可生物降解性等优点，肽通常被用作自组装过程中的构建块。在肽自组装的引发剂中，酶是在温和的反应条件下指导该过程的优秀候选者。碱性磷酸酶（ALP）作为一种重要且常用的生物标志物，能够切割磷酸基团，引发亲水性到疏水性的转变，从而诱导肽自组装。近年来，ALP 指导的肽自组装在生物成像和治疗方面取得了突破，激发了用于诊断和治疗的自组装生物材料的发展。在本文中，我们重点介绍了 ALP 指导的肽组装的最新进展，并对其潜在影响进行了展望。最后，我们简要讨论了该领域未来研究中存在的挑战。
