Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, India.
Indian Council of Agricultural and Research, Fisheries Science Division, Krishi Anusandhan Bhawan II, New Delhi, India.
J Recept Signal Transduct Res. 2024 Apr;44(2):54-62. doi: 10.1080/10799893.2024.2395301. Epub 2024 Aug 26.
Preeclampsia, a gestational associated hypertension, has been reported in 6-8% of pregnant women worldwide leading to premature delivery and low birth weight of newborn due to reduced blood flow to placenta. Although several vasodilators (Methyl dopa, hydralazine, β-blockers and diuretics) are currently in use to treat preeclampsia, still there is a search for safer drugs with better efficacy. Lately, antihypertensive vasodilators from natural sources are gaining importance in treating preeclampsia. Eugenol (Eug), a natural essential oil, has been traditionally used in health and food products without any risk. In the present study, experiments were designed to examine the vasorelaxation effect of Eug and its signaling pathways in a middle uterine artery (MUA) of pregnant (). In presence of different blockers (L-NAME, indomethacin, ODQ, Ouabain, glibenclamide, 4-AP, Ba, Carbenoxolone and 18β Glycyrrhetinic acid), Eug-induced concentration-dependent vasorelaxation response was elicited. The results showed that Eug caused a greater vasorelaxation effect in the MU of pregnant animals, which is mediated by potential activation of eNOS, K channels, and K channels with moderate activation of Na- K- ATPase and sGC and MEGJ. These findings provide a strong basis for developing Eug as a therapeutic candidate in the treatment of pregnancy-associated hypertension.
子痫前期是一种与妊娠相关的高血压，据报道，全球有 6-8%的孕妇患有这种疾病，由于胎盘血流量减少，导致早产和新生儿体重低。尽管目前有几种血管扩张剂（Methyl dopa、hydralazine、β受体阻滞剂和利尿剂）用于治疗子痫前期，但仍在寻找更安全、疗效更好的药物。最近，来自天然来源的降压血管扩张剂在治疗子痫前期方面越来越受到重视。丁香酚（Eug）是一种天然精油，传统上用于保健品和食品中，没有任何风险。在本研究中，设计了实验来研究 Eug 在怀孕（）的子宫中动脉（MUA）中的血管舒张作用及其信号通路。在存在不同阻断剂（L-NAME、吲哚美辛、ODQ、哇巴因、格列本脲、4-AP、Ba、Carbenoxolone 和 18β 甘草次酸）的情况下，Eug 诱导出浓度依赖性的血管舒张反应。结果表明，Eug 在怀孕动物的 MU 中引起更大的血管舒张作用，这是通过潜在激活 eNOS、K 通道和 K 通道介导的，同时适度激活 Na-K-ATP 酶和 sGC 和 MEGJ。这些发现为将 Eug 开发为治疗与妊娠相关的高血压的治疗候选药物提供了强有力的依据。
