Eye Hospital, the First Affiliated Hospital of Harbin Medical University.
Eye Hospital, the First Affiliated Hospital of Harbin Medical University;
J Vis Exp. 2024 Aug 9(210). doi: 10.3791/67127.
Ocular surface squamous neoplasia (OSSN) is the most common tumor of the ocular surface, ranging from mild dysplasia to invasive squamous carcinoma. Traditionally, the diagnosis of OSSN relies on histopathological confirmation followed by a full-thickness biopsy. However, in the past two decades, the therapeutic approach to OSSN has shifted from surgical intervention to topical chemotherapy regimens in clinical settings. This shift emphasizes the need for less invasive or non-invasive methods to diagnose ocular surface pathologies. Among various imaging devices, commercially available high-resolution optical coherence tomography (HR-OCT) has emerged as a powerful tool for the characterization of OSSN. HR-OCT provides an in vivo, cross-sectional view of ocular surface lesions, offering an "optical biopsy" for OSSN with high sensitivity and specificity. It provides valuable information in differentiating intraepithelial or invasive OSSN from other benign lesions. Additionally, HR-OCT can be used to monitor the response to topical chemotherapy and to detect subclinical OSSN during follow-up visits. In this article, the scanning protocol for image acquisition is presented, and image interpretation for OSSN is outlined. This standardized, practical, and reproducible approach is recommended in clinical workflows and is expected to assist clinicians in the management of OSSN.
眼表鳞状上皮肿瘤（OSSN）是最常见的眼表肿瘤，从轻度发育不良到侵袭性鳞状细胞癌不等。传统上，OSSN 的诊断依赖于组织病理学确认，然后进行全层活检。然而，在过去的二十年中，OSSN 的治疗方法已从临床环境中的手术干预转变为局部化疗方案。这种转变强调了需要使用更少侵入性或非侵入性的方法来诊断眼表疾病。在各种成像设备中，市售的高分辨率光学相干断层扫描（HR-OCT）已成为 OSSN 特征描述的强大工具。HR-OCT 提供了眼表病变的体内横截面视图，为 OSSN 提供了高灵敏度和特异性的“光学活检”。它可用于区分上皮内或侵袭性 OSSN 与其他良性病变。此外，HR-OCT 可用于监测局部化疗的反应，并在随访期间检测亚临床 OSSN。本文介绍了图像采集的扫描方案，并概述了 OSSN 的图像解释。这种标准化、实用且可重复的方法在临床工作流程中被推荐，有望帮助临床医生管理 OSSN。
