Goyal Ravinder K, Hui Joseph P M, Ranches Jeffrey, Stefanova Roumiana, Jones Alysson, Banskota Arjun H, Burton Ian, Yu Bianyun, Berrue Fabrice, Hannig Albert, Clark Shawn, Chatterton Syama, Dhaubhadel Sangeeta, Zhang Junzeng
Lacombe Research and Development Centre, Agriculture and Agri-Food Canada, 6000 C and E Trail, Lacombe, AB, T4L 1W1, Canada.
Aquatic and Crop Resource Development Research Centre, National Research Council of Canada, 1411 Oxford St., Halifax, NS, B3H 3Z1, Canada.
Phytopathology. 2024 Dec;114(12):2502-2514. doi: 10.1094/PHYTO-04-24-0151-R. Epub 2024 Dec 6.
In soilborne diseases, the plant-pathogen interaction begins as soon as the seed germinates and develops into a seedling. , an oomycete, stays dormant in soil and is activated by sensing the host through chemical signals present in the root exudates. The composition of plant exudates may, thus, play an important role during the early phase of infection. To better understand the role of root exudates in plant resistance, we investigated the interaction between partially resistant lines (PI660736 and PI557500) and susceptible pea cultivars (CDC Meadow and AAC Chrome) against during the pre-invasion phase. The root exudates of the two sets of cultivars clearly differed from each other in inducing oospore germination. PI557500 root exudate not only had diminished induction but also inhibited the oospore germination. The contrast between the root exudates of resistant and susceptible cultivars was reflected in their metabolic profiles. Data from fractionation and oospore germination inhibitory experiments identified a group of saponins that accumulated differentially in susceptible and resistant cultivars. We detected 56 saponins and quantified 44 of them in pea root and 30 from root exudate; the majority of them, especially soyasaponin I and dehydrosoyasaponin I with potent in vitro inhibitory activities, were present in significantly higher amounts in both roots and root exudates of PI660736 and PI557500 compared with Meadow and Chrome. Our results provide evidence for saponins as deterrents against , which might have contributed to the resistance against root rot in the studied pea cultivars. [Formula: see text] Copyright © 2024 His Majesty the King in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada and the National Research Council of Canada. This is an open access article distributed under the CC BY 4.0 International license.
在土传病害中，植物与病原体的相互作用在种子萌发并发育成幼苗时就开始了。卵菌纲的一种病原菌会在土壤中保持休眠状态，并通过感知根分泌物中存在的化学信号而被激活。因此，植物分泌物的组成在感染早期可能起着重要作用。为了更好地理解根分泌物在植物抗性中的作用，我们研究了部分抗性品系（PI660736和PI557500）与易感豌豆品种（CDC Meadow和AAC Chrome）在侵染前阶段对该病原菌的相互作用。两组品种的根分泌物在诱导卵孢子萌发方面明显不同。PI557500的根分泌物不仅诱导作用减弱，还抑制卵孢子萌发。抗性和易感品种根分泌物之间的差异反映在它们的代谢谱中。分级分离和卵孢子萌发抑制实验的数据确定了一组在易感和抗性品种中差异积累的皂苷。我们在豌豆根中检测到56种皂苷，并对其中44种进行了定量，在根分泌物中检测到30种；与Meadow和Chrome相比，PI660736和PI557500的根和根分泌物中，大多数皂苷，尤其是具有强大体外抑制活性的大豆皂苷I和脱氢大豆皂苷I，含量明显更高。我们的结果为皂苷作为抵御该病原菌的威慑物提供了证据，这可能有助于所研究的豌豆品种抵抗根腐病。[公式：见正文] 版权所有© 2024加拿大女王陛下，由加拿大农业和农业食品部长以及加拿大国家研究委员会代表。这是一篇根据知识共享署名4.0国际许可协议分发的开放获取文章。
