Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Republic of Korea.
Otol Neurotol. 2024 Oct 1;45(9):998-1005. doi: 10.1097/MAO.0000000000004317. Epub 2024 Aug 26.
Memantine, an N -methyl- d -aspartate receptor antagonist, is widely used to treat Alzheimer's disease and has been found to have potential neuroprotective effects. In this study, we evaluated the protective effects of memantine against cisplatin-induced ototoxicity.
Cisplatin is a widely used anticancer drug for various cancers; however, its use is limited by its side effects, including ototoxicity. Several drugs have been developed to reduce cisplatin toxicity. In this study, we treated cisplatin-damaged cochlear hair cells with memantine and evaluated its protective effects.
House Ear Institute Organ of Corti 1 (HEI-OC1) cells and cochlear explants were treated with cisplatin or memantine. Cell viability, apoptotic patterns, reactive oxygen species (ROS) production, Bcl-2/caspase-3 activity, and cell numbers were measured to evaluate the anti-apoptotic and antioxidative effects of memantine.
Memantine treatment significantly improved cell viability and reduced cisplatin-induced apoptosis in auditory cells. Bcl-2/caspase-3 activity was also significantly increased, suggesting anti-apoptotic effects against cisplatin-induced ototoxicity.
Our results suggest that memantine protects against cisplatin-induced ototoxicity in vitro, providing a potential new strategy for preventing hearing loss in patients undergoing cisplatin chemotherapy.
美金刚是一种 N-甲基-D-天冬氨酸受体拮抗剂，广泛用于治疗阿尔茨海默病，并且已被发现具有潜在的神经保护作用。在这项研究中，我们评估了美金刚对顺铂诱导的耳毒性的保护作用。
顺铂是一种广泛用于多种癌症的抗癌药物；然而，由于其副作用，包括耳毒性，其应用受到限制。已经开发了几种药物来降低顺铂的毒性。在这项研究中，我们用美金刚处理顺铂损伤的耳蜗毛细胞，并评估其保护作用。
使用 House Ear Institute Organ of Corti 1 (HEI-OC1) 细胞和耳蜗外植体进行顺铂或美金刚处理。通过测量细胞活力、凋亡模式、活性氧 (ROS) 产生、Bcl-2/caspase-3 活性和细胞数量来评估美金刚的抗凋亡和抗氧化作用。
美金刚处理显著提高了细胞活力并减少了顺铂诱导的听觉细胞凋亡。Bcl-2/caspase-3 活性也显著增加，表明对顺铂诱导的耳毒性具有抗凋亡作用。
我们的结果表明，美金刚可防止体外顺铂诱导的耳毒性，为接受顺铂化疗的患者预防听力损失提供了一种新的潜在策略。
