You Yijun, Li Dan, Chen Zhuangzhuang, Zhang Xiangnan, Hu Yuxuan, Ouyang Shigen, Li Na
Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510006, People's Republic of China.
School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao, 066004, People's Republic of China.
Mikrochim Acta. 2024 Aug 26;191(9):563. doi: 10.1007/s00604-024-06638-0.
A fluorescent and colorimetric dual-mode strategy based on carbon dots (CDs) was rationally designed for sensitive determination of Cu. Green fluorescent CDs with high absolute quantum yield of 72.9% were synthesized by facile one-step hydrothermal treatment of triethylenetetramine and Rose Bengal. Cu could trigger the oxidative and chromogenic reaction of p-phenylenediamine (PPD) to generate chromogenic PPDox, accompanied by the fluorescence quenching of the CDs. The quenching mechanism was identified as the inner filter effect between PPDox and CDs. Therefore, a colorimetric/fluorescent dual-mode detection method for Cu recognition was constructed. The limits of detection for Cu were 4.14 μM and 1.28 μM for colorimetric and fluorescent mode, respectively. In addition, this method had achieved satisfactory results in the detection of Cu in real serum samples.
基于碳点（CDs）设计了一种荧光和比色双模式策略用于灵敏测定铜。通过对三亚乙基四胺和玫瑰红进行简便的一步水热处理，合成了具有72.9%高绝对量子产率的绿色荧光碳点。铜可引发对苯二胺（PPD）的氧化显色反应生成显色产物PPDox，同时伴随碳点荧光猝灭。猝灭机制确定为PPDox与碳点之间的内滤效应。因此，构建了一种用于铜识别的比色/荧光双模式检测方法。比色和荧光模式下铜的检测限分别为4.14 μM和1.28 μM。此外，该方法在实际血清样品中铜的检测中取得了满意结果。
