基于桥联三苯胺的荧光探针用于尿液中 HSA 的选择性和直接检测。

Bridged triphenylamine-based fluorescent probe for selective and direct detection of HSA in urine.

机构信息

Key Laboratory of General Chemistry of the National Ethnic Affairs Commission, College of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China.

Key Laboratory of Pollution Control Chemistry and Environmental Functional Materials for Qinghai - Tibet Plateau of the National Ethnic Affairs Commission, School of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China.

出版信息

Bioorg Chem. 2024 Nov;152:107742. doi: 10.1016/j.bioorg.2024.107742. Epub 2024 Aug 22.

DOI:10.1016/j.bioorg.2024.107742

PMID:39186916

Abstract

Human serum albumin (HSA) serves as a crucial indicator for therapeutic monitoring and biomedical diagnosis. In this study, a near infrared (NIR) fluorescent probe, termed BTPA, characterized a donor-π-acceptor (D-π-A) structure based on bridged triphenylamine (TPA) was developed. BTPA exhibited outstanding sensitivity and selectivity towards HSA among various analysts, with a remarkable 50-fold fluorescence enhancement with a significant Stokes shift (∼190 nm) and a wide linear detection range of 0-20 μM of HSA. Especially, BTPA displayed selectivity for discrimination of HSA from BSA. Job's Plot analysis suggested a 1:1 stoichiometry for the formation of the BTPA-HSA complex. Displacement assays and molecular docking demonstrated that BTPA binds to subdomain IB of HSA which could effectively avoid interference from most drugs. Besides, BTPA have good biocompatibility and could detect of exogenous HSA with a relatively low fluorescence background. For practical applications, BTPA was tested for detecting HSA levels in human urine without any pretreatment, showing detection capability in the range of 0-10 μM with a fast response (<30 s), a limit of detection (LOD) of 0.12 μM and good recoveries (81.7-92.9 %), highlighting the high performance of bridged triphenylamine-based probe BTPA.

摘要

人血清白蛋白（HSA）是治疗监测和生物医学诊断的重要指标。在这项研究中，我们开发了一种近红外（NIR）荧光探针，称为 BTPA，它具有基于桥连三苯胺（TPA）的给体-π-受体（D-π-A）结构。BTPA 在各种分析物中对 HSA 表现出出色的灵敏度和选择性，具有显著的 50 倍荧光增强，显著的斯托克斯位移（∼190nm）和 0-20μM 的宽线性检测范围。特别是，BTPA 对 HSA 与 BSA 的区分具有选择性。Job 的作图分析表明，BTPA-HSA 复合物的形成具有 1:1 的化学计量比。置换实验和分子对接表明，BTPA 结合到 HSA 的亚域 IB，这可以有效地避免来自大多数药物的干扰。此外，BTPA 具有良好的生物相容性，可以检测外源性 HSA，并且具有相对较低的荧光背景。对于实际应用，我们测试了 BTPA 用于检测未经任何预处理的人尿中的 HSA 水平，显示出在 0-10μM 范围内的检测能力，响应时间快（<30s），检测限（LOD）为 0.12μM，回收率良好（81.7-92.9%），突出了基于桥连三苯胺的探针 BTPA 的高性能。