Laboratory of Clinical Pathology, Azienda Sanitaria Universitaria Integrata, Udine, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Flow Cytometry Diagnostic Center and Immunotherapy Unit, Careggi University Hospital, Florence, Italy.
Autoimmun Rev. 2024 Sep;23(9):103604. doi: 10.1016/j.autrev.2024.103604. Epub 2024 Aug 24.
Classification criteria of autoimmune rheumatic diseases are an important means to define homogenous groups of patients that can be compared across studies for clinical trials and research purposes. The measurement of autoantibodies is a relevant aspect in the definition of classification criteria, with a significant weight in the scores necessary to classify patients with autoimmune rheumatic diseases. The impact of autoantibodies has gradually increased over the years, contributing to the evolution and improvement of the classification criteria. However, these criteria often do not take into consideration how autoantibodies are measured, i.e. differences in diagnostic accuracy of the methods. This is a critical point especially when obsolete analytical methods that are no longer used in many clinical laboratories are taken into consideration. In this review we have critically examined assays and methods for the determination of autoantibodies that are (or could be) included among the classification criteria of autoimmune rheumatic diseases in light of more recent evidence and technology evolution.
自身免疫性风湿病的分类标准是定义同质患者群体的重要手段，可以在临床试验和研究目的中进行比较。自身抗体的测量是分类标准定义中的一个相关方面，在用于分类自身免疫性风湿病患者的评分中具有重要意义。自身抗体的影响多年来逐渐增加，有助于分类标准的演变和改进。然而，这些标准通常没有考虑到如何测量自身抗体，即方法的诊断准确性存在差异。当考虑到已经过时且不再许多临床实验室使用的分析方法时，这是一个关键问题。在这篇综述中，我们根据最近的证据和技术发展，批判性地检查了（或可能被）纳入自身免疫性风湿病分类标准的自身抗体的测定和方法。
