Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt 19117, Jordan.
Department of Plant Production and Protection, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt 19117, Jordan.
J Microbiol Biotechnol. 2024 Sep 28;34(9):1848-1856. doi: 10.4014/jmb.2404.04024. Epub 2024 Jul 18.
green microalga offers many environmentally friendly applications, including wastewater treatment, biofertilizers, animal feed, and biofuel production. Different strains of have unique properties that may suit one application but not another. There is a need to distinguish between the many available strains of to choose the one that best fits the application. Consequently, our research goal was to develop strain-specific simple sequence repeat (SSR) markers to differentiate between the different strains. Seventeen markers spanning ten out of the twelve chromosomes of the genome were developed and validated on eight different strains from culture collections and our lab, and were then analyzed by fragment analysis. The results demonstrate the potential of these polymorphic markers to detect the genetic differences between the strains of , and to serve as useful tools for the intra-species population genetic analysis and conservation genetics studies of .
绿微藻具有许多环保应用，包括废水处理、生物肥料、动物饲料和生物燃料生产。不同品系的 具有独特的性质，可能适合一种应用，但不适合另一种应用。需要区分可用的 许多菌株，以选择最适合应用的菌株。因此，我们的研究目标是开发品系特异性简单重复序列 (SSR) 标记，以区分不同的菌株。开发了 17 个标记，跨越 基因组的 12 条染色体中的 10 条，并在来自培养物收集和我们实验室的 8 个不同菌株上进行了验证，然后通过片段分析进行了分析。结果表明，这些多态性标记具有检测菌株之间遗传差异的潜力，并可作为种内群体遗传分析和保护遗传学研究的有用工具。
