Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, P.R. China.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao266109, P.R. China.
J Microbiol Biotechnol. 2024 Sep 28;34(9):1898-1911. doi: 10.4014/jmb.2405.05004. Epub 2024 Jul 24.
is an annual fungus that specifically parasitizes living plants, offering significant potential for developing new resource food and medicine. However, wild resources and mycelium production of this fungus is limited, and its anti-tumor active ingredients and mechanisms remain unclear, hampering the development of this fungus. Thus, we optimized the fermentation medium of and studied the anti-tumor activity of its mycelium. The results indicated that the optimum fermentation medium consisted of 2% sucrose, 0.2% peptone, 0.1% KHPO, 0.05% MgSO·7HO, 0.16% petals, 0.18% P fungal elicitor, and 0.21% stem. The biomass reached 7.82 ± 0.41 g/l after 15 days of cultivation in the optimized medium, a 142% increase compared with the potato dextrose broth medium, with a 64% reduction in cultivation time. The intracellular alcohol extract had a higher inhibitory effect on A549 and Eca-109 cells than the intracellular water extract, with half-maximal inhibitory concentration values of 2.42 and 2.92 mg/ml, respectively. Graded extraction of the alcohol extract yielded petroleum ether phase, chloroform phase, ethyl acetate phase, and n-butanol phase. Among them, the petroleum ether phase exhibited a better effect than the positive control, with a half-maximal inhibitory concentration of 113.3 μg/ml. Flow cytometry analysis indicated that petroleum ether components could induce apoptosis of Eca-109 cells, suggesting that this extracted component can be utilized as an anticancer agent in functional foods. This study offers valuable technical support and a theoretical foundation for promoting the comprehensive development and efficient utilization of .
是一种寄生在活体植物上的一年生真菌，具有开发新型资源食品和药物的巨大潜力。然而，该真菌的野生资源和菌丝体产量有限，其抗肿瘤活性成分和机制尚不清楚，限制了该真菌的发展。因此，我们优化了 的发酵培养基，并研究了其菌丝体的抗肿瘤活性。结果表明，最佳发酵培养基由 2%蔗糖、0.2%蛋白胨、0.1% KHPO、0.05% MgSO·7HO、0.16% 花瓣、0.18% P 真菌诱导剂和 0.21% 茎组成。在优化后的培养基中培养 15 天后，生物量达到 7.82±0.41 g/L，比土豆葡萄糖肉汤培养基提高了 142%，培养时间缩短了 64%。与胞内水提物相比，胞内醇提物对 A549 和 Eca-109 细胞的抑制作用更高，半数最大抑制浓度值分别为 2.42 和 2.92 mg/ml。醇提物的分级萃取得到石油醚相、氯仿相、乙酸乙酯相和正丁醇相。其中，石油醚相的效果优于阳性对照，半数最大抑制浓度为 113.3 μg/ml。流式细胞术分析表明，石油醚成分可诱导 Eca-109 细胞凋亡，提示该提取成分可作为功能性食品中的抗癌药物。本研究为 的综合开发和高效利用提供了有价值的技术支持和理论基础。
