蒙古马卵泡发育过程中卵泡液的蛋白质组学和代谢组学特征。

Proteome and metabolomic profile of Mongolian horse follicular fluid during follicle development.

机构信息

College of Animal Science, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.

College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266000, China.

出版信息

Sci Rep. 2024 Aug 26;14(1):19788. doi: 10.1038/s41598-024-66686-7.

DOI:10.1038/s41598-024-66686-7

PMID:39187528

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11347562/

Abstract

During follicular development, changes in the composition of the follicular fluid are synchronized with the development of oocytes. Our aim was to screen the key factors affecting oocyte maturation and optimize the in vitro culture protocol by understanding the changes of proteins and metabolites in follicular fluid. Follicles are divided into three groups according to their diameter (small follicle fluid (SFF): 10 mm < d < 20 mm; medium follicle fluid (MFF): 20 mm < d < 30 mm; large follicle fluid (LFF): 30 mm < d). Proteins and metabolites from the follicular fluid were analyzed by mass spectrometry. The results showed that: in LFF vs MFF, 20 differential abundant protein (DAP) and 88 differential abundant metabolites (DAM) were screened out; In SFF vs MFF, 3 DAPs and 65 DAMs were screened out; In MFF vs SFF, 24 DAPs and 35 DAMs were screened out. The analysis of differential proteins and metabolites showed that glycerophosphate hydrolysis decreased during follicular development, and proteins played a major role in metabolism and binding. In addition, DAMs and DAPs are co-enriched in the "linoleic acid metabolism" pathway. Combinatorial analysis reveals the dynamic profile of follicular fluid during follicular development and provides fundation for further exploring the function of follicular fluid in Mongolian horse.

摘要

在卵泡发育过程中，卵泡液的成分变化与卵母细胞的发育同步。我们的目的是通过了解卵泡液中蛋白质和代谢物的变化，筛选出影响卵母细胞成熟的关键因素，并优化体外培养方案。根据卵泡直径将卵泡分为三组（小卵泡液（SFF）：10mm＜d＜20mm；中卵泡液（MFF）：20mm＜d＜30mm；大卵泡液（LFF）：30mm＜d）。采用质谱法分析卵泡液中的蛋白质和代谢物。结果表明：在 LFF 与 MFF 相比中，筛选出 20 个差异丰度蛋白（DAP）和 88 个差异丰度代谢物（DAM）；在 SFF 与 MFF 相比中，筛选出 3 个 DAP 和 65 个 DAM；在 MFF 与 SFF 相比中，筛选出 24 个 DAP 和 35 个 DAM。差异蛋白和代谢物分析表明，甘油磷酸水解在卵泡发育过程中减少，蛋白质在代谢和结合中起主要作用。此外，DAM 和 DAP 共同富集在“亚油酸代谢”途径中。组合分析揭示了卵泡液在卵泡发育过程中的动态特征，为进一步探索卵泡液在蒙古马中的功能提供了基础。