Fujiwara M, Kariyone A, Shiraki M
Clin Exp Immunol. 1985 Jan;59(1):161-8.
In order to clarify role of lpr gene of MRL/Mp-lpr/lpr (MRL/l) mice in the development of immunological abnormalities and lupus nephritis, F2 mice were obtained from crosses of (MRL/1 X AKR) F1 mice and examined with regard to lymph node enlargement, numbers of immunoglobulin (IgM and IgG) producing cells (PC) in the spleen, serum levels of anti-trinitrophenyl (TNP) and anti-DNA antibodies, amounts of circulating immune complexes (IC) and histopathological findings of the kidney. Out of total numbers of 234 F2 mice, 54 mice (F2[+]mice) (23.1%) were with lymphoproliferation and 180 mice (F2[-]mice) (76.9%) without it. As parental controls, 39 MRL/1, 27 AKR and 21 F1 mice were also checked. There were significant differences in the all of the parameters tested between F2 (+) and F2 (-) mice. Significant differences were also observed between F2 (+) and MRL/l mice in the weight of mesenteric lymph nodes, numbers of IgGPC, levels of IgG antibodies and amounts of IC, but not in the numbers of IgMPC and levels of IgM antibodies. All of F1 mice tested mimicked AKR mice in the values of various parameters described above. In MRL/l and F2 (+) mice, there were no significant correlations between the weight of mesenteric lymph nodes and other parameters. These data seem to show that the lpr gene behaves as a single recessive trait and there exists gene(s) which suppresses expression of the lpr gene.
为了阐明MRL/Mp-lpr/lpr（MRL/l）小鼠的lpr基因在免疫异常和狼疮性肾炎发展中的作用，通过（MRL/1×AKR）F1小鼠杂交获得F2小鼠，并对其淋巴结肿大、脾脏中产生免疫球蛋白（IgM和IgG）的细胞（PC）数量、抗三硝基苯（TNP）和抗DNA抗体的血清水平、循环免疫复合物（IC）的量以及肾脏的组织病理学发现进行了检查。在总共234只F2小鼠中，54只小鼠（F2[+]小鼠）（23.1%）出现淋巴细胞增殖，180只小鼠（F2[-]小鼠）（76.9%）未出现。作为亲本对照，还检查了39只MRL/1、27只AKR和21只F1小鼠。F2（+）和F2（-）小鼠之间在所有测试参数上均存在显著差异。在肠系膜淋巴结重量、IgGPC数量、IgG抗体水平和IC量方面，F2（+）和MRL/l小鼠之间也观察到显著差异，但在IgMPC数量和IgM抗体水平方面没有差异。所有测试的F1小鼠在上述各种参数值上都与AKR小鼠相似。在MRL/l和F2（+）小鼠中，肠系膜淋巴结重量与其他参数之间没有显著相关性。这些数据似乎表明lpr基因表现为单一隐性性状，并且存在抑制lpr基因表达的基因。
