Liang Si-Zhou, Chang Ya-Jun, Semaha Philip, Liu Li-Zhu, Gao Yan, Wang Zhi, Zhang Wei-Guo
China Ministry of Agriculture Key Laboratory at Yangtze River Plain for Agricultural Environment, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Water Res X. 2024 Jul 26;24:100241. doi: 10.1016/j.wroa.2024.100241. eCollection 2024 Sep 1.
Given that many organic pollutants have been reported to facilitate the plasmid-mediated conjugative transfer of antibiotic resistance genes (ARGs), it was naturally deduced that nonylphenol (NP) can also have this kind of effect. Whereas, this study demonstrates an entirely different result that environmentally relevant concentrations of NP attenuate plasmid-mediated ARGs conjugative transfer (maximum inhibition rate 64 %), further study show that NP exposure had no significant effect on bacterial growth, cell vitality, oxidative stress response, and expression of conjugation-relevant genes, which were reported to closely relate to the conjugative transfer in numerous studies. Conclusively, it was found that the dispersant function of NP impeded the occurrence of cell mating, thus was responsible for the decline of conjugative transfer. This study shows a new perspective on understanding the effect of organic pollutants like NP on the ARGs horizontal dissemination in environment.
鉴于已有报道称许多有机污染物可促进质粒介导的抗生素抗性基因（ARGs）的接合转移，因此自然推断壬基酚（NP）也可能具有这种作用。然而，本研究证明了一个完全不同的结果，即环境相关浓度的NP会减弱质粒介导的ARGs接合转移（最大抑制率64%），进一步研究表明，NP暴露对细菌生长、细胞活力、氧化应激反应以及在众多研究中被报道与接合转移密切相关的接合相关基因的表达均无显著影响。最终发现，NP的分散作用阻碍了细胞配对的发生，从而导致接合转移的下降。本研究为理解像NP这样的有机污染物对环境中ARGs水平传播的影响提供了一个新的视角。
