Wen Cai, Shuanghong Yang, Wanrong Hu, Ran Chen, Zhishun Chai, Danqun Huo, Changjun Hou, Dongliang Li, Qianying Zhang
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongging University, Chonging, China.
Cigar Fermentation Technology Key Laboratory of China Tobacco, China Tobacco Sichuan Industrial Co., Ltd., China Tobacco Technology Innovation Center for Cigar, Chengdu, China.
Front Bioeng Biotechnol. 2024 Aug 12;12:1440961. doi: 10.3389/fbioe.2024.1440961. eCollection 2024.
To investigate the effects of different media and media addition methods on the fermentation of tobacco. A plant extract and a bacterial agent are used for fermenting cigar tobacco leaves in a different order of addition. The chemical composition, cellulose, and pectin content, as well as changes in the microbial community, were measured. The addition method of the fermentation medium affects the quality of fermented tobacco. The optimal medium formula and addition method involve first adding plant extracts and then microbial agents. The medium formula and addition method can significantly reduce cellulose in tobacco, with a reduction rate of 46%, and significantly increase the content of β-carotene, thereby enhancing the aroma of cigarettes. There is an increase in aroma components, such as alcohols, alkanes, and olefins, in tobacco. By reducing the proportion of Aspergillus, it can alter the microbial community structure of tobacco. Adding plant extracts before introducing microbial agents can significantly improve the quality and alter the microbial community structure of Dexue No.1 tobacco.
为研究不同培养基及培养基添加方式对烟草发酵的影响。采用一种植物提取物和一种菌剂，以不同的添加顺序对雪茄烟叶进行发酵。测定了化学成分、纤维素和果胶含量以及微生物群落的变化。发酵培养基的添加方式影响发酵烟草的品质。最佳培养基配方及添加方式为先添加植物提取物，再添加微生物菌剂。该培养基配方及添加方式可显著降低烟草中的纤维素，降低率达46%，并显著提高β-胡萝卜素含量，从而提升卷烟香气。烟草中醇类、烷烃类和烯烃类等香气成分增加。通过降低曲霉比例，可改变烟草的微生物群落结构。在引入微生物菌剂之前添加植物提取物可显著提高德雪一号烟草的品质并改变其微生物群落结构。
