Department of Stomatology, The Fourth Affiliated Hospital of Soochow University, Suzhou Dushu Lake Hospital, Medical Center of Soochow University, Suzhou, China.
Fujian Key Laboratory of Oral Diseases and Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
Front Cell Infect Microbiol. 2024 Aug 12;14:1361373. doi: 10.3389/fcimb.2024.1361373. eCollection 2024.
Temporomandibular joint disorders (TMD) are highly prevalent among people. Numerous investigations have revealed the impact of gut microbiota in many diseases. However, the causal relationship between Temporomandibular joint disorders and gut microbiota remains unclear.
Genome-Wide Association Studies (GWAS) refer to the identification of sequence variations, namely single nucleotide polymorphisms (SNPs), existing across the entire human genome. GWAS data were collected on gut microbiota and TMD. Then, instrumental variables were screened through F-values and removal of linkage disequilibrium. These SNPs underwent mendelian analysis using five mathematical models. Sensitivity analysis was conducted to further verify the stability of the results. Pathogenic factors of TMD mediate the causal relationship between gut microbiota and TMD were explored through a two-step Mendelian randomization analysis. Finally, reverse mendelian analysis was conducted to account for potential reverse effects.
The analysis of the data in this article suggests that some gut microbiota, including Coprobacter, Ruminococcus torques group, Catenibacterium, Lachnospiraceae, Turicibacter, Victivallis, MollicutesRF9, Methanobacteriales, Methanobacteriaceae, FamilyXI, Methanobacteria were identified as risk factors, while Peptococcaceae provides protection for TMD.
The research reveals the relation of gut microbiota in TMD. These findings provide insights into the underlying mechanisms and suggest potential therapeutic strategy.
颞下颌关节紊乱（TMD）在人群中高发。大量研究揭示了肠道微生物群在许多疾病中的作用。然而，TMD 与肠道微生物群之间的因果关系尚不清楚。
全基因组关联研究（GWAS）是指识别整个人类基因组中存在的序列变异，即单核苷酸多态性（SNP）。GWAS 数据收集了肠道微生物群和 TMD 方面的数据。然后，通过 F 值和去除连锁不平衡来筛选工具变量。这些 SNP 采用了五种数学模型进行孟德尔分析。通过敏感性分析进一步验证结果的稳定性。通过两步孟德尔随机化分析来探索 TMD 的致病因素在肠道微生物群与 TMD 之间的因果关系。最后，进行反向孟德尔分析以考虑潜在的反向效应。
本文数据分析表明，一些肠道微生物群，包括柯氏菌、罗氏梭菌群、双歧杆菌、真杆菌科、瘤胃球菌科、Turicibacter、Victivallis、 MollicutesRF9、甲烷杆菌目、甲烷杆菌科、科 X1、甲烷杆菌，被确定为危险因素，而消化球菌科为 TMD 提供保护。
本研究揭示了 TMD 中肠道微生物群的关系。这些发现为潜在的治疗策略提供了深入的机制见解。
