Corretto Erika, Trenti Massimiliano, Štarhová Serbina Liliya, Howie James Malcolm, Dittmer Jessica, Kerschbamer Christine, Candian Valentina, Tedeschi Rosemarie, Janik Katrin, Schuler Hannes
Faculty of Agricultural, Environmental and Food Sciences, Free University of Bozen-Bolzano, Bozen-Bolzano, Italy.
Competence Centre for Plant Health, Free University of Bozen-Bolzano, Bozen-Bolzano, Italy.
J Pest Sci (2004). 2024;97(3):1299-1314. doi: 10.1007/s10340-023-01699-1. Epub 2023 Oct 6.
Phytoplasmas are bacterial pathogens located in the plant's phloem that are responsible for several plant diseases and are mainly transmitted by phloem-sucking insects. Apple proliferation (AP) is an economically important disease associated with the presence of ' Phytoplasma mali' which is transmitted by two psyllid species. While is a vector in different regions, the vector efficiency of varies between different populations. This species is considered the main AP vector in Northwestern Italy but plays a minor role in Northeastern Italy and other European regions. To investigate whether the psyllid and/or the phytoplasma subtype drive the phytoplasma acquisition in , a phytoplasma acquisition experiment was set up using single mating couples of overwintered individuals from different psyllid populations and phytoplasma subtypes. All analyzed insect populations acquired phytoplasma, but with different efficiencies and concentrations. The main factors driving the acquisition were the phytoplasma subtype and its concentration in the leaves of the infected trees together with the psyllid lineage. The phytoplasma concentration in the psyllids was again influenced by the phytoplasma subtype, the psyllid lineage and the region of origin, whereas the phytoplasma concentration in the leaves and the psyllid haplotype defined with the cytochrome oxidase I gene had only a minor impact on the phytoplasma concentration. This is the first study evaluating the roles of both the psyllid haplotype and the phytoplasma subtype on the acquisition process and highlights the importance of as an additional AP vector.
The online version contains supplementary material available at 10.1007/s10340-023-01699-1.
植原体是存在于植物韧皮部的细菌病原体，可引发多种植物病害，主要通过吸食韧皮部的昆虫传播。苹果增殖病（AP）是一种与“苹果植原体”相关的具有经济重要性的病害，由两种木虱传播。虽然 在不同地区是传播媒介，但不同种群的 传播效率有所不同。该物种在意大利西北部被认为是主要的苹果增殖病传播媒介，但在意大利东北部和其他欧洲地区作用较小。为了研究木虱和/或植原体亚型是否驱动了 在 中的植原体获取，利用来自不同木虱种群和植原体亚型的越冬个体单配成对进行了一项植原体获取实验。所有分析的昆虫种群都获取了植原体，但效率和浓度不同。驱动获取的主要因素是植原体亚型及其在感染树木叶片中的浓度以及木虱谱系。木虱体内的植原体浓度再次受到植原体亚型、木虱谱系和起源地区的影响，而叶片中的植原体浓度以及用细胞色素氧化酶I基因定义的木虱单倍型对植原体浓度的影响较小。这是第一项评估木虱单倍型和植原体亚型在获取过程中作用的研究，并强调了 作为苹果增殖病额外传播媒介的重要性。
在线版本包含可在10.1007/s10340-023-01699-1获取的补充材料。
