Many surgical procedures in a soft tissue are performed using diodes lasers. This study aimed to assess the temperature rise and pain following the use of 810 and 980 nm diode lasers for second-stage dental implant surgery. This clinical trial was conducted on 24 osseointegrated dental implants that were randomly divided into two groups of 810 nm and 980 nm diode lasers. The temperature rise in each group was measured right after uncovering by the laser and 15 minutes later by a thermocouple, compared with the baseline temperature of gingival tissue. The level of pain was also measured at 24 hours postoperatively by using a visual analog scale. Data were analyzed by ANOVA, Tukey's test, and test (alpha=0.05). Within-group comparisons by ANOVA showed a significant difference in tissue temperature between the three time points in both groups (<0.0001). Pairwise comparisons by Tukey's test showed that the temperature at baseline (<0.0001) and 15 seconds after uncovering was significantly lower than that immediately after uncovering in both groups (<0.0001). The mean tissue temperature and the mean pain score in the 980 nm laser group were significantly higher than the corresponding values in the 810 nm laser group (<0.05). According to the results, temperature rise in the use of the 980 nm laser was higher than the 810 nm laser. The use of 810 nm diode laser was associated with lower temperature rise and significantly lower pain score after 24 hours.