School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore.
Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
Anal Methods. 2024 Sep 19;16(36):6164-6172. doi: 10.1039/d4ay00982g.
The growing awareness of the health benefits associated with probiotics has led to an increasing interest in probiotic products. To develop probiotic functional foods that deliver health benefits, it is essential to characterize both probiotic viability (the ability to survive) and vitality (the ability to remain active and effective). However, traditional probiotic assays only provide limited information about their survival state. To gain a comprehensive understanding of probiotic states, a D-amino-acid-based metabolic labeling strategy was applied to quantitatively depict probiotic vitality. In this approach, probiotics were first metabolically incorporated with azido-modified D-lysine and then labeled with dibenzocyclooctyne-sulfo-Cy5 through click chemistry. This two-step labeling process provides a visual representation of the metabolic levels of probiotics as well as the bacterial membrane integrity. Besides, this method is capable of characterizing the influence of various environmental conditions, from manufacturing to oral administration, on probiotic vitality. With its rapid detection process and general applicability, this strategy has the potential to be widely implemented in the food industry for probiotic vitality evaluation.
人们对益生菌相关健康益处的认识不断提高，导致人们对益生菌产品的兴趣日益浓厚。为了开发具有健康益处的益生菌功能性食品，必须对益生菌的生存能力（存活能力）和活力（保持活性和有效性的能力）进行特征描述。然而，传统的益生菌检测方法只能提供有关其存活状态的有限信息。为了全面了解益生菌的状态，应用了基于 D-氨基酸的代谢标记策略来定量描述益生菌的活力。在这种方法中，益生菌首先与叠氮修饰的 D-赖氨酸进行代谢结合，然后通过点击化学与二苯并环辛炔磺基-Cy5 进行标记。这种两步标记过程提供了益生菌代谢水平以及细菌膜完整性的直观表示。此外，该方法能够描述从制造到口服给药等各种环境条件对益生菌活力的影响。由于其快速的检测过程和普遍适用性，该策略有可能在食品工业中广泛用于益生菌活力评估。
