Yamarthi Prathyusha, Kotipalli Rama Satyasri, Patnaik Samatasai, Veena Kv, Kathirvel Muralidharan, Vutukuri Rajkumar, Bhanoori Manjula
Department of Biochemistry, Osmania University, Hyderabad 500007, India.
Applied Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India.
Pathophysiology. 2024 Aug 15;31(3):408-419. doi: 10.3390/pathophysiology31030031.
The present study aims to evaluate the effect of ink on insulin resistance in PCOS-induced mice. Treatment with sepia ink in dehydroepiandrosterone (DHEA)-induced PCOS mice at various doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight mitigated the insulin resistance in the study groups with decreased concentration of testosterone and increased concentrations of estrogen and progesterone compared to the PCOS group tested by ELISA. The histopathological analysis and restoration of glucose analysis showed a significant reduction in treatment groups. Reduced expression of insulin resistance genes like androgen receptor (AR), insulin receptor substrate 1 (IRS-1), and insulin-like growth factor1 (IGF-1) by qRT-PCR indicate a positive impact of sepia ink in alleviating the symptoms associated with PCOS. Taken together, the results of this study indicate sepia ink as a promising therapeutic intervention and a possible drug target for insulin resistance in diabetes and gynecological disorders like PCOS.
本研究旨在评估乌贼墨对多囊卵巢综合征（PCOS）诱导的小鼠胰岛素抵抗的影响。通过酶联免疫吸附测定法（ELISA）检测，与PCOS组相比，用不同剂量（50毫克/千克、100毫克/千克和200毫克/千克体重）的乌贼墨处理脱氢表雄酮（DHEA）诱导的PCOS小鼠，可减轻研究组的胰岛素抵抗，同时降低睾酮浓度，提高雌激素和孕酮浓度。组织病理学分析和葡萄糖分析结果显示，治疗组有显著改善。通过实时定量聚合酶链反应（qRT-PCR）检测发现，雄激素受体（AR）、胰岛素受体底物1（IRS-1）和胰岛素样生长因子1（IGF-1）等胰岛素抵抗相关基因的表达降低，表明乌贼墨对缓解PCOS相关症状有积极作用。综上所述，本研究结果表明，乌贼墨有望成为治疗糖尿病和PCOS等妇科疾病中胰岛素抵抗的治疗干预手段和潜在药物靶点。
