Nofal Amany Ebrahim, Shaaban Amal Mohamed, Ibrahim Hany Mohammed, Abouelmagd Faten, Mohamed Azza Hassan
Histology and Histochemistry Unit, Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom 32511, Egypt.
Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom 32511, Egypt.
J Xenobiot. 2024 Jul 29;14(3):1003-1022. doi: 10.3390/jox14030056.
Bilharziasis is a widespread trematode parasite that poses a severe public health burden. Dandelion () has several pharmacological and traditional properties critical for treating several hepatic disorders. The present study was designed to assess the potential efficacy of root (TOR) dietary supplementation with or without praziquantel (PZQ) against liver and intestinal disorders in mice infected with . This study was conducted on five groups; G1: uninfected control, G2: untreated -infected mice, G3: infected animals treated with 250 mg/kg PZQ for three alternative days, G4: infected animals were orally administered 600 mg/kg bw TOR daily for 10 days, and G5: infected animals that received both PZQ and TOR as previously described. The current findings after different treatments indicated topographical scanning electron microscopy alterations of male adult worms and a critical reduction in worm burden, ova count, granuloma diameter, hepatic and intestinal histological abnormalities, fibrosis, immunohistochemical expression of CD3 and CD20 cells, oxidative stress, and interleukin-10, also upregulation of interferon-gamma, and antioxidant enzymes, when compared to the infected untreated mice. The best results were obtained in mice administered PZQ+TOR together because of their antioxidant properties and ability to promote the host immune response to parasitic infection.
血吸虫病是一种广泛传播的吸虫寄生虫，给公共卫生带来了沉重负担。蒲公英具有多种药理特性和传统药用价值，对治疗多种肝脏疾病至关重要。本研究旨在评估在感染曼氏血吸虫的小鼠中，单独或联合使用吡喹酮（PZQ）进行蒲公英根（TOR）膳食补充对肝脏和肠道疾病的潜在疗效。本研究共分为五组：G1：未感染对照组；G2：未治疗的感染小鼠；G3：感染动物每隔一天接受250 mg/kg PZQ治疗，共治疗三天；G4：感染动物每天口服600 mg/kg体重的TOR，持续10天；G5：感染动物按照上述方法同时接受PZQ和TOR治疗。不同处理后的当前研究结果表明，与未治疗的感染小鼠相比，雄性成虫的扫描电子显微镜形态发生改变，虫负荷、虫卵计数、肉芽肿直径、肝脏和肠道组织学异常、纤维化、CD3和CD20细胞的免疫组化表达、氧化应激以及白细胞介素-10均显著降低，同时干扰素-γ和抗氧化酶上调。联合使用PZQ和TOR的小鼠取得了最佳结果，这归因于它们的抗氧化特性以及促进宿主对寄生虫感染免疫反应的能力。
