定向选择实验与酶多态性：黑腹果蝇G6PD基因座上辛酸选择的负面证据。
Targeted selection experiments and enzyme polymorphism: negative evidence for octanoate selection at the G6PD locus in Drosophila melanogaster.
作者信息
Eanes W F, Bingham B, Hey J, Houle D
出版信息
Genetics. 1985 Feb;109(2):379-91. doi: 10.1093/genetics/109.2.379.
Abstract
Published studies have reported significant selection with respect to the G6pd locus for Drosophila melanogaster reared on Na-octanoate food. We have reexamined the selective effects of Na-octanoate on egg to adult viability with respect to the G6pd polymorphism using specially constructed X chromosomes. Four experiments were carried out using different 6Pgd backgrounds in two recombinant sets of chromosomes segregating for the G6pd locus but constructed so as to minimize variation over most of the X chromosome. In addition, two measures of viability were used, and the size of the experiments and their associated degrees of freedom are approximately double those reported in the former studies. Our results find no evidence for differential selection on G6pd genotypes (males and females) by Na-octanoate and, therefore, do not corroborate the positive results of selection reported by other investigators. The reasons for our different results are discussed.
摘要
已发表的研究报告称，在以辛酸钠为食饲养的黑腹果蝇中，G6pd基因座存在显著的选择作用。我们使用特制构建的X染色体，重新研究了辛酸钠对G6pd多态性从卵到成虫存活率的选择效应。在两组不同的重组染色体中进行了四项实验，这些染色体因G6pd基因座而分离，但构建方式是为了尽量减少大部分X染色体上的变异。此外，还使用了两种存活率测量方法，实验规模及其相关自由度约为先前研究报告的两倍。我们的结果未发现辛酸钠对G6pd基因型（雄性和雌性）有差异选择的证据，因此，并未证实其他研究人员所报告的选择阳性结果。文中讨论了我们得出不同结果的原因。
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