Bijlsma R, Kerver J W
Genetics. 1983 Mar;103(3):447-64. doi: 10.1093/genetics/103.3.447.
For the degradation of DDT and other chlorohydrocarbon insecticides energy in the form of NADPH is needed which for the greater part is supplied by the pentose phosphate shunt. Therefore the influence of DDT on the polymorphism at the G6pd and Pgd loci in Drosophila melanogaster was investigated by studying its effect on egg to adult survival and adult survival. The results show the existence of significant differences in fitness between the different genotypes of the two loci for both components. It is found that the effect of DDT supplementation differs significantly from the effect of sodium octanoate addition. DDT treatment also increases the activity of the pentose phosphate shunt as measured by the activity of G6PD and 6PGD. In larvae a 50% increase in activity is found and in adults a 100% increase. As there is little doubt that the activities of G6PD and 6PGD are somehow correlated with the fitness of flies, the data are discussed in relation to the in vitro and in vivo differences in activity between the different allozymes of both G6PD and 6PGD.
对于滴滴涕及其他氯代烃类杀虫剂的降解而言，需要以烟酰胺腺嘌呤二核苷酸磷酸（NADPH）形式存在的能量，其中大部分由磷酸戊糖途径提供。因此，通过研究滴滴涕对果蝇从卵到成虫存活以及成虫存活的影响，来探究其对黑腹果蝇葡萄糖-6-磷酸脱氢酶（G6pd）和6-磷酸葡萄糖酸脱氢酶（Pgd）基因座多态性的影响。结果表明，对于这两个组成部分，两个基因座的不同基因型之间在适合度上存在显著差异。研究发现，添加滴滴涕的效果与添加辛酸钠的效果存在显著差异。通过测量葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）的活性可知，滴滴涕处理还会增加磷酸戊糖途径的活性。在幼虫中，活性增加了50%，在成虫中，活性增加了100%。由于几乎可以肯定葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）的活性在某种程度上与果蝇的适合度相关，因此将结合葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）不同同工酶在体外和体内活性的差异来讨论这些数据。
