Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Immunology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Scand J Immunol. 2024 Nov;100(5):e13402. doi: 10.1111/sji.13402. Epub 2024 Aug 27.
Hepatitis B (HB) infection is a major global health problem. There is limited knowledge about HB vaccination-induced immune memory responses. We compared the frequency of CD8 memory T cell subsets between responders (RSs) and non-responders (NRs) to HB vaccination. Blood samples were collected from RSs and NRs. PBMCs were cultured in the presence of Hepatitis B surface antigens (HBsAg) and PHA for 48 h to restimulate CD8 memory T cells and T cell memory subsets were detected by flow cytometry using memory cell markers. The frequency of T, T, and T was significantly higher in responders compared to non-responders (p = 0.024, 0.022, and 0.047, respectively). Additionally, we report a positive correlation between the frequency of T cells in RSs with age and anti-HBsAb level (p = 0.03 and r = 0.5; p = 0.01 and r = 0.06). Responders display a higher level of CD8 T cell-mediated immunity. Therefore, we suggest a possible defect in the formation of immunological CD8 memory T cells in NRs and it may reduce antibody production compared to the RSs, although more experiments are needed.
乙型肝炎（HB）感染是一个全球性的主要健康问题。关于乙型肝炎疫苗接种诱导的免疫记忆反应的知识有限。我们比较了乙型肝炎疫苗接种应答者（RS）和无应答者（NR）之间的 CD8 记忆 T 细胞亚群的频率。从 RS 和 NR 采集血样。将 PBMC 在乙型肝炎表面抗原（HBsAg）和 PHA 的存在下培养 48 小时以重新刺激 CD8 记忆 T 细胞，并通过使用记忆细胞标志物的流式细胞术检测 T 细胞记忆亚群。与无应答者相比，应答者的 T、T 和 T 频率明显更高（p=0.024、0.022 和 0.047）。此外，我们报告了 RS 中 T 细胞频率与年龄和抗-HBsAb 水平之间的正相关（p=0.03 和 r=0.5；p=0.01 和 r=0.06）。应答者显示出更高水平的 CD8 T 细胞介导的免疫。因此，我们建议在 NR 中可能存在免疫 CD8 记忆 T 细胞形成的缺陷，与 RS 相比，这可能会减少抗体产生，尽管还需要更多的实验。
